Phytochemical profiling using HR-LCMS and evaluation of the in vitro antioxidant and antidiabetic potential of hydroalcoholic extract of Ipomoea cairica leaves
"HR-LCMS Profiling and Antioxidant, Antidiabetic Evaluation of Ipomoea cairica Leaf Extract"
Keywords:
Anti diabetic, DPPH, Superoxide, Alpha Glucosidase, HR-LCMSAbstract
ABSTRACT:
Aim: This paper examines the phytochemical composition and biological functions of Ipomoea cairica leaf hydroalcoholic extract (HEIC), which is a perennial herb traditionally used in the treatment of inflammation, diarrhea and febrile rashes.
Material and Methods: A range of bioactive compounds was detected with the help of High-Resolution Liquid Chromatography-Mass Spectrometry (HR-LCMS), among which flavonoid and alkaloid compounds, phenolic acids, and glycosides are well-known as antioxidants and antidiabetic.
Results: HEIC in vitro antioxidant capacity was assessed using a series of assays which showed that the solution has a dose-dependent radical-scavenging activity, but the activity of this compound is lower than the one of ascorbic acid. More so, the extract had moderate inhibitory effects on alpha-amylase and alpha-glucosidase enzymes with IC50 values of 130.67 µg/mL and 212 µg/mL, respectively, suggesting its possible clinical use in the control of postprandial glucose concentration.
Discussion and Conclusion: Major phytochemicals, including quercetin 3-rhamanoside-7-glucoside, rutin, physalis K, and 1,4-Di-O-caffeoylquinic acid, were associated with the therapeutic value of the extract. These results support conventional uses of Ipomoea cairica in herbal medicine and emphasize its potential in preparation of natural antioxidants, and antidiabetic agents.
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