ASSESSING THE BIOACTIVITY AND ANTIOXIDATIVE PROPERTIES OF SOME COMPOUNDS ISOLATED FROM ABUTILON HIRTUM (LAM.)
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i3.16229Abstract
ABSTRACT
Objectives: The present study on the methanol extract of Abutilon hirtum (Lam.) [Malvaceae] afforded ten compounds. Findings from this assessment
indicated that A. hirtum leaves possessed vast potential as medicinal product especially in liver cancer treatment.
Methods: Dried-powdered leaves were boiled under reflux in 10 L of petroleum ether for 8 hrs. After filtration, the solvent was evaporated; afforded
15 g of petroleum ether extract followed by boiling with reflux for 8 hrs with chloroform, then filtration and the residue was evaporated to give 34 g
chloroform extract. Ethyl acetate was added and refluxed for 8 hrs, then filtration and evaporation to give 31 g ethyl acetate extract. Finally the leaves
were refluxed with 10 L of 85 % aqueous MeOH, after cooling, the solution was filtered and evaporated and the residue was 210 g methanol extract
then the residue was dissolved in de-ionized water (250 ml), then the salt was removed by adding excess methanol solution (2.5 L), and finally filtered.
The cytotoxicity of the isolated compounds was evaluated towards HepG2 liver-carcinoma cell line using MTT assay, the antimicrobial activity was
tested using the Disc agar plate method and the total antioxidant capacity was determined according to phosphomolybdenum method.
Results: The isolated compounds identified as methyl gallate, cuneataside E, bergapten, gallic acid, ellagic acid, epigallocatechin-3-O-gallate,
kaempferol-3-O-α-L-rhamnoside, benzyl-1-O-β-D-glucopyranoside, 2(4-hydroxyphenyl)ethyl-O-β-D-glucopyranoside and β-sitosterol. All the isolated
compounds are known; but they were isolated from Abutilon hirtum for the first time.
Conclusion: This report may serve as a footprint concerning the biological and pharmacological activities of A. hirtum leaves.
Keywords: Abutilon hirtum, Malvaceae, HepG2, Phenolics, Antioxidant.
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