ISOLATION AND SCREENING OF L-ASPARAGINASE PRODUCING SPHINGOMONAS SP. FROM SOIL SAMPLE

DEEPAK VMM; VAISHNAVI  L; RADHA PALANISWAMY

doi:10.22159/ajpcr.2025v18i3.53842

Authors

DEEPAK VMM Department of Biotechnology, CMS College of Science and Commerce, Coimbatore, Tamil Nadu, India https://orcid.org/0009-0006-8215-3204
VAISHNAVI L Department of Biotechnology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India
RADHA PALANISWAMY Department of Biotechnology, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India https://orcid.org/0000-0002-1793-0103

DOI:

https://doi.org/10.22159/ajpcr.2025v18i3.53842

Keywords:

Sphingomonas leidyi, L-asparaginase, Anticancer, Acute lymphoblastic leukemia

Abstract

Objectives: In recent years, L-asparaginase has gained attention due to its anticancer activity. Different types of asparaginase possess different characteristics and different applications. The study is designed to screen potential L-asparaginase producing bacteria from soil samples.

Methods: Soil samples were collected from different locations in Tamil Nadu and Kerala for the isolation of L-Asparaginase-producing bacteria.

Results: About 689 isolates were obtained from 114 samples. Among these, 214 isolates possess the ability to synthesize L-asparaginase. Fifteen isolates were selected for quantitative screening based on the intensity of the pink color formation M9 basal medium.

Conclusion: The isolate VN01 exhibited a higher degree of enzyme activity (156 IU/mg). The isolate was further categorized by phenotypic and genotypic method and the isolate was identified as Sphingomonas leidyi. As a future aspect, the study will be continued for optimization of increased production of L-asparaginase and in characterizing the potential of L-asparaginase synthesized by S. leidyi.

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