TO STUDY THE EFFECT OF MONOSODIUM GLUTAMATE ON THE URINARY BLADDER
DOI:
https://doi.org/10.22159/ajpcr.2025v18i5.54229Keywords:
Aspartame, Swiss Albino mice, Urinary bladder, HistologyAbstract
Objective: This study evaluates the appropriateness of statistical tests (Z-test, t-test, and Mann–Whitney U test) for small-sample analyses, addressing common misconceptions and providing evidence-based guidelines for test selection in low-data scenarios.
Methods: A comparative framework was developed to assess the performance of parametric and non-parametric tests under varying sample sizes (n<10 to n=30) and distributional assumptions (normal vs. skewed). Normality was tested through Shapiro–Wilk tests, and simulated datasets with controlled variance and outliers were analyzed.
Results: Z-tests produced inflated type I errors for n<30 due to reliance on known population variance, a rarely feasible assumption in small samples. T-tests maintained robustness for n≥15 with normal distributions, whereas Mann–Whitney U tests outperformed parametric alternatives for n<15 or non-normal data (skewness >2).
Conclusion: Researchers should default to t-tests for small samples with approximate normality and use Mann–Whitney U tests for highly skewed data or n<15. Transparent reporting of sample sizes, normality checks, and test rationale is critical to ensure methodological validity.
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References
Young DB, McCormick GM 2nd. There’s death in the cup, so beware! Am J Forensic Med Pathol. 1995;16(3):223-8. doi: 10.1097/00000433- 199509000-00007. PMID 7495263
Lajtha A, Reilly MA, Dunlop DS. Aspartame consumption: Lack of effects on neural function. J Nutr Biochem. 1994;5(6):266-83. doi: 10.1016/0955-2863(94)90032-9
Parthasarathy NJ, Kumar RS, Manikandan S, Devi RS. Methanol-induced oxidative stress in rat lymphoid organs. J Occup Health. 2006;48(1):20-7. doi: 10.1539/joh.48.20, PMID 16484759
Wurtman RJ. Neurochemical changes following high-dose aspartame with dietary carbohydrates. N Engl J Med. 1983;309(7):429-30. doi: 10.1056/NEJM198308183090710, PMID 6877300
Kitahori Y, Kitamura M, Konishi N, Matsuda H, Tao M, Matsui E, et al. Carcinogenicity study of monosodium aspartate in Fischer 344 rats: 100 weeks treatment. J Toxicol Pathol. 1996;9(2):161-8. doi: 10.1293/ tox.9.161
Umi BI. The Effect of Aspartame to the Serum Creatinine Level and Histologic Structure of Kidney in Mice. Available from: http://26068. blogspot.com/2007/05/effect aspartame.htm
World Health Organization. Evaluation of Certain Food Additives and Contaminants. World Health Organization; 2016.
Mukhopadhyay M, Mukherjee A, Chakrabarti J. In vivo cytogenetic studies on blends of aspartame and acesulfame-K. Food Chem Toxicol. 2000;38(1):75-7. doi: 10.1016/s0278-6915(99)00115-5, PMID 10685017
Abdollahi M. Mechanism of aspartame induced antinociception in mice. Indian J Pharmacol. 2003;35:37-41.
Martins MR, Azoubel R. Effects of aspartame on fetal kidney: A orphometric and stereological study. Int J Morphol 2007;25:689-94. doi: 10.4067/s0717-95022007000400004
AlSuhaibani ES. In vivo cytogenetic studies on aspartame. Comp Funct Genomics. 2010;2010:605921. doi: 10.1155/2010/605921, PMID 20689731
Iman I. Effect of aspartame on some oxidative stress parameters in liver and kidney of rats. Afr J Pharm Pharmacol. 2011;5(6):678-862. doi: 10.5897/AJPP10.302
Li H, Liang H, Yang H, Zhang X, Ding X, Zhang R, et al. Association between intake of sweetened beverages with all-cause and cause-specific mortality: A systematic review and meta-analysis. J Public Health (Oxf). 2022;44(3):516-26. doi: 10.1093/pubmed/fdab069, PMID 33837431
Potts WJ. SC -18862: A Sweetening Agent. Pharmacological Studies. WHO Food Additives Series; 1973. p. 15.
Ghanem AM. A review on recent advances in transdermal drug delivery systems of tamsulosin. Int J Appl Pharm. 2024;16(2):28-33. doi: 10.22159/ijap.2024v16i2.49950
Jagadeesh K, Kadiyala KG, Varma Dendukuri BN, Guttula RS, Gupta Tiruveedhi VL, et al. Quality control assessment of dutasteride and silodosin in capsules and tablets employing a novel developed HPLC technique; evaluation of stabilities of dutasteride and silodosin in accelerated degradation. Int J Appl Pharm. 2023;15(6):98-107. doi: 10.22159/ijap.2023v15i6.49036
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Copyright (c) 2025 Vijay Gujar, Karuna Kachhawa, Mohan Pethe, Sanket Hiware, Aditya Tarnekar, Sameer Yelwatkar
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