PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITIES OF GYMNEMA SYLVESTRE R. BR. STEM AND LEAF EXTRACTS
DOI:
https://doi.org/10.22159/ajpcr.2025v18i8.54874Keywords:
Gymnema sylvestre, High-performance liquid chromatography, 1,1-diphenyl-2-picryl hydrazine assay, AntioxidantAbstract
Objective: This study of aims to analyze Phytochemical analysis from Gymnema sylvestre leaf and stem extract and their antioxidant capability.
Methods: The methanol extract was subjected to both qualitative and quantitative evaluations utilizing standard protocols.Fresh plant leaf extracts are used for qualitative phytochemical analysis to find the presence of the different phytoconstituents. High-performance liquid chromatography was used for quantitative phytochemical analysis, where as the 1,1-diphenyl-2-picryl hydrazine (DPPH)-free radical technique was used for antioxidant activity. It has been demonstrated that the phytochemical components of medicinal plants are mostly responsible for their antioxidant qualities.
Results: Antioxidant activity of methanolic leaf extract of Gymnema sylvestre R.Br. Might be due to the presence of Cardiac glycoside,Phenols,Saponins,Glycoside, found in the preliminary phytochemical analysis. The dried leaves were extracted under continuous hot extraction in soxhlet apparatus with 90% methanol gave the gymnemic acid(0.02 %), Gymnemagenin (0.61%), Deacyl gymnemic acid(2.50%) by using HPLC methods. Percentage of Inhibition increases with increasing concentration, showing free radical scavenging activity. At 1000 μg/ml, GSM shows 82.97% inhibition,while ascorbic acid shows 89.08% inhibition at 100μg/ml.
Conclusion: G. sylvestre leaf methanol extract exhibits strong antioxidant properties.
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