EVALUATION OF HEPATOPROTECTIVE POTENTIAL OF APOCYNACEAE FAMILY PLANTS IN RAT MODELS OF ALCOHOL, PARACETAMOL, AND RANITIDINE-INDUCED HEPATOTOXICITY

SUBBA RAO CHAMAKURI; ASHISH SUTTEE; PRASENJIT MONDAL; DASARI PRIYANKA

doi:10.22159/ajpcr.2025v18i8.55364

Authors

SUBBA RAO CHAMAKURI Department of Pharmacognosy, School of Pharmaceutical Sciences, Lovely Professional University, Jalandhar, Punjab, India.
ASHISH SUTTEE Department of Pharmacognosy, School of Pharmaceutical Sciences, Lovely Professional University, Jalandhar, Punjab, India. https://orcid.org/0000-0003-3539-281X
PRASENJIT MONDAL Department of Pharmaceutical Technology, Brainware University, West Bengal, Kolkata, India
DASARI PRIYANKA Department of Pharmacology, JNTUH, Vaageswari College of Pharmacy, Karimnagar, Telangana, India

DOI:

https://doi.org/10.22159/ajpcr.2025v18i8.55364

Keywords:

Plumeria pudica, Hepatoprotective, Paracetamol, Ethanol,, Ranitidine,, Liver injury

Abstract

Objectives: This study aimed to evaluate the hepatoprotective activity of the methanolic leaf extract of Plumeria pudica (PP) against hepatotoxicity induced by Paracetamol, ethanol, and ranitidine in rat models.

Methods: The presence of key bioactive constituents, such as saponins and flavonoids in the methanolic extract of PP leaves was confirmed through gas chromatography-mass spectrometry analysis. Rats were divided into six groups (n=6/group). Hepatotoxicity was induced using Paracetamol, ethanol, and ranitidine. Silymarin served as the standard reference drug. The extract was administered at various doses, and hepatoprotective activity was assessed in comparison with the control group.

Results: Treatment with PP extract significantly reduced the levels of aspartate transaminase, alanine transaminase, serum total bilirubin, total protein, triglycerides, and cholesterol when compared to the toxicant-treated group (Group II). Histopathological examination revealed reduced hepatic necrosis and inflammation, supporting the biochemical findings.

Conclusion: The methanolic leaf extract of PP demonstrated significant (p<0.05*, p<0.01**, p<0.001***) hepatoprotective effects in rat models of drug- and alcohol-induced liver injury. These effects are attributed to the presence of saponins and flavonoids, which may contribute to membrane stabilization and antioxidant properties.

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References

1. Padh H, Patel B. Herbal drugs. Curr Sci. 2001;81:15.

2. Thaibinh T. Herbal medication. Indian J Pharm Educ. 1998;32:104-6.

3. Zimmerman HJ, Maddrey WC. Acetaminophen (paracetamol) hepatotoxicity with regular intake of alcohol: Analysis of instances of therapeutic misadventure. Hepatology. 1995;22(3):767-73. Hepatology’s Erratum 1995;22(6):1898.

4. Bounous DI, Jones DP, Brackett BG, Rzucidlo SJ. Acute acetaminophen toxicity in transgenic mice with elevated hepatic glutathione. Vet Hum Toxicol. 2000;42(3):146-50.

5. Handa SS, Sharma A, Chakraborti KK. Natural products and plants as liver protecting drugs. Fitoterapia. 1986;57:307-51.

6. Dienstag JL, Isselbacher KJ. Harrison’s principles of internal medicine 2001. In: Toxic and Drug-Induced Hepatitis. 15th ed., Vol. 2., Ch. 296. United States: McGraw-Hill Companies, Incorporated; p. 1737-42.

7. Bhave SA, Pandit AN, Pradhan AM, Sidhaye DG, Kantarjian A, Williams A, et al. Liver disease in India. Arch Dis Child. 1982;57(12):922- 8. doi: 10.1136/adc.57.12.922, PMID 7181521

8. Devasagayam TP, Vaidya AD. Current status of herbal drugs in India: An overview. J Clin Biochem Nutr. 2007;41(1):1-11. doi: 10.3164/ jcbn.2007001

9. Herfindal ET, Gourley DR. 6th ed. Casebook for herfindal and Gourley’s textbook of therapeutics: Drug and disease management. United States: Williams and Wilkins; 1996. p. 543-4.

10. Kausik P, Lalkhokra DS, Sharma C, Aneja KR, Khan S. Madhuca indica anti-oxidant and antibacterial testing. Pharmacology. 2010;2:1-8.

11. Chatterjee TK. Hepatoprotective herbs. In: Herbal Options. 3rd ed. Calcutta: Books and Allied (P) Ltd.; 2000. p. 155.

12. Suttee A, Mondal P, Chamakuri SR. An eye-catching and comprehensive review on Leucas zeylanica (Ceylon slitwort). Int J Res Pharm Sci. 2020;11(SPL4):2932-8.

13. Valsaraj R, Pushpangadan P, Smitt UW, Adsersen A, Nyman U. Antimicrobial screening of selected medicinal plants from India. J Ethnopharmacol. 1997;58(2):75-83.

14. Das SN, Patro VJ, Dinda SC. A review: Ethnobotanical survey of genus Leucas. Pharmcogn Rev. 2012;6(12):100-10.

15. Napagoda M, Gerstmeier J, Butschek H, Lorenz S, Kanatiwela D, Qader M, et al. Lipophilic extracts of Leucas zeylanica, a multi-purpose medicinal plant in the tropics, inhibit key enzymes involved in inflammation and gout. J Ethnopharmacol. 2018;224:474-81.

16. Duthie MS, Kimber I, Norval M. The effects of ultraviolet radiation on the human immune system. Br J Dermatol. 1999;140(6):995-1009.

17. Manoranjan T, Thangarajah R, Thavaranjit AC. Antifungal activity and qualitative phytochemical analysis of some medicinal plants in Jaffna (Sri Lanka). Int J Org Chem. 2018;8(4):335-40.

18. Swetha B. Methanolic Leucas zeylanica leaf extract antioxidant activity frog heart isolation. Sour Innov J Sci. 2019;7(1):1-3.

19. Hung NH, Chuong NT, Satyal P, Hieu HV, Dai DN, Huong LT, et al. Mosquito larvicidal activities and chemical compositions of the essential oils of Leucas zeylanica growing Wild in Vietnam. Nat Prod Commun. 2020;14(6):1-7.

20. Sujatha V, Kathirvel A. Phytochemical studies, antioxidant activities and identification of active compounds using GC-MS of Dryopteris cochleata leaves. Arab J Chem. 2016;9:1435-42.

21. Paulsamy S, Karthika K, Senguttuvan J. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. For in vitro antioxidant activities. Asian Pac J Trop Biomed. 2014;4 Suppl 1:S359-67.

22. Ministry of Social Justice and Empowerment. OECD Chemical Testing Guidelines, Revised Draft 423: Acute Oral Toxicity-Acute Toxic Class Method. India: CPCSEA. Ministry of Social Justice and Empowerment; 2001.

23. Ganapaty S, Dash GK, Subburaju T, Suresh P. Diuretic, laxative and toxicity studies of Cocculus hirsutus aerial parts. Fitoterapia. 2018;73:28-31.

24. Shivhare Y, Dangi P, Soni P, Singh P, Baghel SS. Acute toxicity study of aqueous extract of Coccinia indica (roots). Asian Pharm Res. 2011;1(1):23-5.

25. Gujrati V, Patel N, Venkat Rao N, Nandakumar K, Gauda TS, Shalam M, et al. Hepatoprotective activity of alcoholic and aqueous extracts of leaves of Tylophora indica (Linn.) in rats. Indian J Pharmacol. 2007;39(1):43-7.

26. Vogel HG. Drug Discovery and Evaluation. 2nd ed. New York: Springer Verlag; 2002. p. 941-2.

27. Vogel WH. Drug Discovery and Evaluation-Pharmacological Assays. 2nd ed., Vol. 406-407. Berlin Heidelberg, Germany: Springer-Verlag; 1997.

28. Hemieda FA, Abdel-Hady SK, Elnga MA. Biochemical and histological studies on H2-receptor antagonist ranitidine-induced hepatotoxicity in rats. Indian J Exp Biol. 2005;43:782-5.

29. Kandimalla R, Dash S, Bhowal AC, Kalita S, Talukdar NC, Kundu S, et al. Glycogen-gold nanohybrid escalates the potency of silymarin. Int J Nanomedicine. 2017;12:7025-38.

30. Kandimalla R, Dash S, Kalita S, Choudhury B, Malampati S, Kalita K, et al. Bioactive guided fractions of Annona reticulata L. Bark: Protection against liver toxicity and inflammation through inhibiting oxidative stress and proinflammatory cytokines. Front Pharmacol. 2016;7:168.

31. Kandimalla R, Dash S, Kalita S, Choudhury B, Malampati S, Kalita K, et al. Protective effect of bioactivity guided fractions of Ziziphus jujuba mill. Root bark against hepatic injury and chronic inflammation via inhibiting inflammatory markers and oxidative stress. Front Pharmacol. 2016;7:298.

32. Yusan LY, Subagio H. Anti-bacterial activity of chitosan nanoparticles gel from crab shell waste (Portunus pelagicus) against Staphylococcus aureus and Escherichia coli bacteria. Int J Appl Pharm. 2025;17(3):343-7.

33. Syahputra A, Ervina I, Darwis AF, Syahputra H, Marpaung ES, Sitorus IJ, et al. In vitro antimicrobial activity of Punica granatum L. Extract and anti-inflammatory activity of nanogel in vivo in wistar rats gingiva. Int J Appl Pharm. 2025;17(3):189-99.

34. Amin S, Parle A. Synthesis, characterization and evaluation of novel 2-aryl benzothiazole derivatives as potential antibacterial agents. Int J Curr Pharm Rev Res. 2025;8(5):342-9.

35. Shinde Nilesh N, Chaitanya K, Ganapathy, Onkar CK. Pharmacovigilance: A global key to drug safety monitoring. Int J Curr Res. 2025;17(3):32142-6.

36. Thakurdesai P, Karve M, Deshpande P. Prenatal developmental toxicity study of polyphenols-based standardized cinnamon bark extract in rats. Int J Pharm P