GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM NEEM BARK FOR THE REMEDIATION OF WATERBORNE PATHOGENS
DOI:
https://doi.org/10.22159/ajpcr.2026v19i2.55385Keywords:
Silver, Nanoparticles,, Neem, Azadirachta, Biosynthesis,, Green, Antibacterial, Antipathogenic,, Larvicidal, Water Purification, Vector,, Ecofriendly, Stability, Crystalline, Characterization, Pathogens, Mosquito, SustainabilityAbstract
Objective: The objective of the study was to develop an eco-friendly method for synthesizing silver nanoparticles (AgNPs) using neem (Azadirachta indica) bark extract as a reducing and stabilizing agent, and to evaluate their antibacterial, antipathogenic, and larvicidal activities for water purification and vector control.
Methods: AgNPs were synthesized by green chemistry using neem bark (NB) extract, avoiding hazardous chemicals. Synthesis parameters – pH, temperature, and reaction time – were optimized. Characterization included ultraviolet (UV)-Visible spectroscopy (Shimadzu UV-1800), Fourier transform infrared (Perkin Elmer Spectrum-One, USA), X-ray diffraction (Cu Kα radiation, λ = 1.5406 Å), SEM (Hitachi S-3400N), TEM (JEOL-JEM- 2100F, 200 kV), particle size analysis, and zeta potential measurement. Antibacterial activity against Staphylococcus aureus and Escherichia coli was tested by the well diffusion method. Antipathogenic activity was assessed using pathogenic sewer water, and larvicidal activity was evaluated against mosquito larvae (n=10/concentration).
Results: Spherical NB AgNPs (40–150 nm) with a characteristic SPR peak at 425 nm were obtained. X-ray diffraction confirmed a face-centered cubic crystalline structure with an average crystallite size of 7.66 nm. Zeta potential values (–26.7 to –33.2 mV) indicated good colloidal stability. Antibacterial testing showed maximum inhibition zones of 47 mm for S. aureus and 37 mm for E. coli at 25 μg/mL. In pathogenic water, smaller particles exhibited higher inhibition efficiency. Larvicidal assays achieved 100% mortality at 2.5 μg/mL within 3 h (p<0.05), with no mortality in controls.
Conclusion: NB -mediated AgNPs demonstrate strong antibacterial, antipathogenic, and larvicidal properties, making them a low-cost, sustainable option for water purification and mosquito control. Further studies should focus on in vivo performance, environmental safety, and large-scale application.
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References
1. World Health Organization. Drinking-water [Internet]. Geneva: WHO; 2023 Sep 13. [cited May 26 2025] Available from: https://www.who. int/news-room/fact-sheets/detail/drinking-water.
2. Melkani I, Kumar B, Pandey NK, Singh S, Baghel DS, Sudhakar K. Therapeutic impact of nanomedicine for the treatment of neuropathic pain: principle, prospective and future. Int J Appl Pharm. 2024;16(5):46-58. doi: 10.22159/ijap.2024v16i5.50457.
3. Nie P, Zhao Y, Xu H. Synthesis, applications, toxicity and toxicity mechanisms of silver nanoparticles: a review. Ecotoxicol Environ Saf. 2023;253:114636. doi: 10.1016/j.ecoenv.2023.114636, PMID 36806822.
4. Shahzadi S, Fatima S, Ul Ain Q, Shafiq Z, Janjua MR. A review on green synthesis of silver nanoparticles (SNPs) using plant extracts: a multifaceted approach in photocatalysis, environmental remediation, and biomedicine. RSC Adv. 2025;15(5):3858-903. doi: 10.1039/ d4ra07519f, PMID 39917042.
5. Asimuddin M, Shaik MR, Adil SF, Siddiqui MR, Alwarthan A, Jamil K et al. Azadirachta indica based biosynthesis of silver nanoparticles and evaluation of their antibacterial and cytotoxic effects. J King Saud Univ Sci. 2020;32(1):648-56. doi: 10.1016/j.jksus.2018.09.014.
6. Kitimu SR, Kirira P, Abdille AA, Sokei J, Ochwang’i D, Mwitari P et al. Anti-angiogenic and anti-metastatic effects of biogenic silver nanoparticles synthesized using Azadirachta indica. Adv Biosci Biotechnol. 2022;13(4):188-206. doi: 10.4236/abb.2022.134010.
7. Ansari M, Ahmed S, Abbasi A, Khan MT, Subhan M, Bukhari NA, et al. Plant mediated fabrication of silver nanoparticles, process optimization, and impact on tomato plant. Sci Rep. 2023;13(1):18048. doi: 10.1038/s41598-023-45038-x, PMID 37872286.
8. Nayak D, Ashe S, Rauta PR, Kumari M, Nayak B. Bark extract mediated green synthesis of silver nanoparticles: evaluation of antimicrobial activity and antiproliferative response against osteosarcoma. Mater Sci Eng C Mater Biol Appl. 2015;58:44-52. doi: 10.1016/j. msec.2015.08.022, PMID 26478285.
9. Bibi S, Abu-Dieyeh MH, Al-Ghouti MA. Biosynthesis of silver nanoparticles from macroalgae Hormophysa triquetra and investigation of its antibacterial activity and mechanism against pathogenic bacteria. Sci Rep. 2025;15(1):2476. doi: 10.1038/s41598-024-84760-y, PMID 39833251.
10. Muhaimin M, Chaerunisaa AY, Rostinawati T, Amalia E, Hazrina A, Nurhasanah S. A review on nanoparticles of Moringa oleifera extract: preparation, characterization, and activity. Int J Appl Pharm. 2023;15(4):43-51. doi: 10.22159/ijap.2023v15i4.47709.
11. Tareq SM, Boutchuen A, Roy S, Zimmerman D, Jur G, Bathi JR, et al. A dynamic light scattering approach for detection of nanomaterials in Tennessee River. Water Resour Res. 2021;57(8):WR028687:e2020. doi: 10.1029/2020WR028687.
12. Antony E, Sathiavelu M, Arunachalam S. Synthesis of silver nanoparticles from the medicinal plant Bauhinia acuminata and Biophytum sensitivum – a comparative study of its biological activities with plant extract. Int J Appl Pharm. 2016;9(1):22-9. doi: 10.22159/ ijap.2017v9i1.16277.
13. Kamble S, Govind K Lohiya, Priyanka Sharnangat, Monika Kherade, Tirupati Rasala, Janhavi Indurkar, et al. Investigating the anti-cancer properties of a newly synthesized metal-quercetin nanocomplex in the context of cervical cancer. Asian J Pharm Clin Res. Apr 2025;18(4):223-9. doi: 10.22159/ajpcr.2025v18i4.53670.
14. Sevinc-Sasmaz C, Erci F, Torlak E, Yöntem M. Characterization of silver nanoparticles synthesized using Hypericum perforatum L. and their effects on Staphylococcus aureus. Microsc Res Tech. 2025 Mar 23;88(8):2321-32. doi: 10.1002/jemt.24862, PMID 40121669.
15. Kaurani P, Hindocha AD, Jayasinghe RM, Pai UY, Batra K, Price C. Effect of addition of titanium dioxide nanoparticles on the antimicrobial properties, surface roughness and surface hardness of polymethyl methacrylate: a Systematic Review. F1000Res. 2023;12:577. doi: 10.12688/f1000research.130028.1, PMID 37424742.
16. Punz B, Christ C, Waldl A, Li S, Liu Y, Johnson L, et al. Nano-scaled advanced materials for antimicrobial applications – mechanistic insight, functional performance measures, and potential towards sustainability and circularity. Environ Sci Nano. 2025;12(3):1710-39. doi: 10.1039/ D4EN00798K.
17. Dass K, Mariappan P, Ramya P, Prakash N. Green synthesis of silver nanoparticles for mosquito control: a review of larvicidal activity from plant extracts. UP J Zool. 2024;45(22):242-66. doi: 10.56557/ upjoz/2024/v45i224677.
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