COMPREHENSIVE PHYTOCHEMICAL ANALYSIS AND THERAPEUTIC EVALUATION OF ACALYPHA FRUTICOSA LEAVES IN TYPE I DIABETIC RATS
DOI:
https://doi.org/10.22159/ajpcr.2026v19i4.58745Keywords:
Acalypha fruticosa, Diabetic prevalence, Type I diabetes mellitus, Antioxidant, Inflammation-related cytokines, Liver health, Alphaamylase glycogenAbstract
Objectives: The study evaluates the safety, phytochemical composition, and effectiveness of Acalypha fruticosa leaf extracts in managing Type I diabetes in rats.
Methods: The methodology involves collecting and verifying plant materials, preparing methanolic extracts, identifying phytochemicals, and determining morphological characteristics. Protective effects are assessed through monitoring body weight, feed, and water consumption, measuring plasma glucose and insulin levels, evaluating lipid peroxidative markers, assessing antioxidant activities, measuring inflammation-related cytokines, assessing liver health, and evaluating the impact on alpha-amylase glycogen levels. Histopathological study is also conducted to observe structural changes and protective effects. The aim is to provide a comprehensive evaluation of the efficacy and safety of A. fruticosa leaves extracts in managing Type I diabetes.
Results: Methanolic extract of A. fruticosa (MeAF) (A. fruticosa) was effective in preventing weight loss, with the higher dose (500 mg/kg) being particularly effective in maintaining body weight. MeAF 250 mg/kg moderately prevented weight loss and improved insulin levels, while MeAF 500 mg/kg significantly reduced blood glucose levels and improved various biomarkers in a dose-dependent manner. MeAF increased catalase activity and restored superoxide dismutase and glutathione levels, showing strong antioxidant potential similar to insulin. MeAF reduced inflammatory markers and showed moderate liver protection, with the higher dose being more effective in reducing alkaline phosphatase, serum glutamate pyruvate transaminase, and serum glutamic oxaloacetic transaminase levels. STZ-induced diabetic rats showed a significant elevation in serum α-amylase levels, whereas treatment with MeAF exhibited a dose-dependent reduction. Similarly, MeAF improved liver glycogen levels and restored plasma vitamin C, with 500 mg/kg producing effects comparable to those observed with insulin. MeAF preserved pancreatic structure with no significant damage to islets or acini at the higher dose, suggesting protective effects against diabetes-induced damage.
Conclusion: A. fruticosa (MeAF) has shown promising potential in managing diabetes and its complications. It improves glucose metabolism, reduces oxidative stress, and provides moderate protection to pancreatic and liver tissues in diabetic rats. MeAF could serve as a natural, complementary therapy, especially in areas with limited access to conventional treatments.
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References
1. American Diabetes Association. Standards of medical care in diabetes. J Clin Appl Res Educ. 2024;47(1):1-328.
2. Rahmani AH, Alsahli MA, Khan AA, Almatroodi SA. Quercetin, a plant flavonol attenuates diabetic complications, renal tissue damage, renal oxidative stress and inflammation in streptozotocin-induced diabetic rats. Metabolites. 2023;13(1):130. doi: 10.3390/metabo13010130, PMID 36677055
3. Piya MK, Tahrani AA, Barnett AH. Emerging treatment options for type 2 diabetes. Br J Clin Pharmacol. 2010;70(5):631-44. doi: 10.1111/j.1365-2125.2010.03711.x, PMID 20831513
4. Rochette L, Zeller M, Cottin Y, Vergely C. Diabetes, oxidative stress and therapeutic strategies. Biochim Biophys Acta. 2014;1840(9):2709-29. doi: 10.1016/j.bbagen.2014.05.017, PMID 24905298
5. Chen J, Liu W, Yi H, Hu X, Peng L, Yang F. The natural rotenoid deguelin ameliorates diabetic neuropathy by decreasing oxidative stress and plasma glucose levels in rats via the Nrf2 signalling pathway. Cell Physiol Biochem. 2018;48(3):1164-76. doi: 10.1159/000491983, PMID 30045011
6. Gahamanyi N, Munyaneza E, Dukuzimana E, Tuyiringire N, Pan CH, Komba EV. Ethnobotany, ethnopharmacology, and phytochemistry of medicinal plants used for treating human diarrheal cases in Rwanda: A review. Antibiotics (Basel). 2021;10(10):1231. doi: 10.3390/ antibiotics10101231, PMID 34680811
7. Chandorkar N, Tambe SM, Amin PD, Madankar CS. A systematic and comprehensive review on current understanding of the pharmacological actions, molecular mechanisms, and clinical implications of the genus Eucalyptus. Phytomed Plus. 2021;1(4):100089. doi: 10.1016/j. phyplu.2021.100089
8. Atanasov AG, Zotchev SB, Dirsch VM, International Natural Product Sciences Taskforce, Supuran CT. Natural products in drug discovery: Advances and opportunities. Nat Rev Drug Discov. 2021;20(3):200-16. doi: 10.1038/s41573-020-00114-z, PMID 33510482
9. Acalypha Fruticose. Available from: https://en.wikipedia.org/wiki/ acalypha_fruticosa
10. Acalypha Fruticose. Available from: https://tropical.theferns.info/ viewtropical.php?id=acalypha+fruticosa
11. Vivekanandarajah S, Vinujan S, Rajamanoharan PR, Sebastian PR. Pharmacological activities of extracts and isolated compounds of Acalypha fruticosa Forssk. Int J Second Metab. 2022;9(3):290-304.
12. Kumar MS, Bhuvaneshwari S, Santhoshkumar M, Arunnesh A, Ameen MM, Sathiyapriya K. A comprehensive review on - Acalypha indica linn. J Pharm Res Int. 2021;33(60B):1631-38. doi: 10.9734/ jpri/2021/v33i60B34787
13. Haimed Y, Sharma PK, Jha DK. Anti-diabetic activity of the methanolic extract of seeds of Syzygium cumini in STZ induced type I diabetes in rats. Int J Pharm Sci Res. 2022;13(8):3333-42.
14. Vb A, Jha DK, Bhattacharjee S. Global trends and burden of diabetes: A comprehensive review of global insights and emerging challenges. Curr J Appl Sci Technol. 2025;44(7):134-50. doi: 10.9734/cjast/2025/ v44i74580
15. Morais AM, Alves A, Kumla D, Morais RM. Pharmaceutical and biomedical potential of sulphated polysaccharides from algae. In: Oliveira JM, Radhouani H, Reis RL, editors. Polysaccharides of Microbial Origin. Cham: Springer; 2022. p. 893-920. doi: 10.1007/978- 3-030-42215-8_49
16. Choudhury A, Jha DK, Rajashekhar U. Anti-inflammatory activity of methanolic extract of Ficus hispida dried fruit. Int J Basic Clin Pharmacol. 2021;10(8):997-1004. doi: 10.18203/2319-2003. ijbcp20212930
17. Nagaraj H, Cyriac KS, Jha DK. Assessment of anti-inflammatory properties of methanolic root extract of Plumeria alba Linn.: An in vitro and in vivo approach. Int J Pharm Investig. 2025;15(4):1175-83. doi: 10.5530/ijpi.20250149
18. Jha D, Koneri R, Samaddar S. Momordica dioica: A review. Int J Pharm Sci Rev Res. 2017 Jul-Aug;45(2):203-9.
19. Aguirre-Crespo FJ, Aragón-Gastélum JL, Gutiérrez-Alcántara EJ, Zamora-Crescencio P, Gómez-Galicia DL, Alatriste-Kurzel DR. β-sitosterol mediates gastrointestinal smooth muscle relaxation induced by Coccoloba uvifera via muscarinic acetylcholine receptor subtype 3. Sci Pharm. 2024;92(2):19. doi: 10.3390/scipharm92020019
20. Al-Daihan S, Al-Faham M, Al-shawi N, Almayman R, Brnawi A, Zargar S. Antibacterial activity and phytochemical screening of some medicinal plants commonly used in Saudi Arabia against selected pathogenic microorganisms. J King Saud Univ Sci. 2013;25(2):115-20. doi: 10.1016/j.jksus.2012.11.003
21. Ferdous RU, Islam A, Tasnim F, Abdullah N, Alam TT, Islam SF. Qualitative phytochemical screenings of methanol extract of Parmentieracereifera Seem fruits. UK J Pharm Biosci. 2014;2(3):3.
22. Shanmugalingam V, Vivekanandarajah S, Rajamanoharan P. Pharmacological activities of extracts and isolated compounds of Acalypha fruticosa Forssk. (Euphorbiaceae). Int J Second Metab. 2022;9(3):290-304. doi: 10.21448/ijsm.1006238
23. Furman BL. Streptozotocin-induced diabetic models in mice and rats. Curr Protoc. 2021;1(4):e78. doi: 10.1002/cpz1.78, PMID 33905609
24. Jha DK, Koneri R, Samaddar S. Antidiabetic activity of phytosaponin in STZ-induced type I diabetes in rats. Res J Pharm Technol. 2019;12(8):3919-26. doi: 10.5958/0974-360X.2019.00675.9
25. Duh PD, Yen GC, Yen WJ, Chang LW. Antioxidant effects of water extracts from barley (Hordeum vulgare L.) prepared under different roasting temperatures. J Agric Food Chem. 2001;49(3):1455-63. doi: 10.1021/jf000882l, PMID 11312880
26. Umre R, Ganeshpurkar A, Ganeshpurkar A, Pandey S, Pandey V, Shrivastava A. In vitro, in vivo and in silico antiulcer activity of ferulic acid. Fut J Pharm Sci. 2018;4(2):248-53. doi: 10.1016/j. fjps.2018.08.001
27. Okado-Matsumoto A, Fridovich I. Subcellular distribution of superoxide dismutases (SOD) in rat liver: Cu,Zn-SOD in mitochondria. J Biol Chem. 2001;276(42):38388-93. doi: 10.1074/jbc.M105395200, PMID 11507097
28. Haimed YA, Sharma PK, Jha DK, Sharma J. A comparative study of polyherbal plants for the management of streptozotocin-induced diabetes in rats. Lat Am J Pharm. 2023;42(3):1257-70.
29. Ali AA, Rajashekhar U, Jha DK. An investigation of type II anti-diabetic activity of biologically active phytochemical(s) of Coccinia indica fruits in high sugar diet-induced diabetic rats. Int J Pharm Sci Drug Res. 2024;16(1):52-8. doi: 10.25004/IJPSDR.2024.160108
30. Biradar S, Veeresh B. Protective effect of lawsone on L-arginine induced acute pancreatitis in rats. Indian J Exp Biol. 2013;51(3):256- 61. PMID 23678547
31. Saxena A, Vikram NK. Role of selected Indian plants in management of type 2 diabetes: A review. J Altern Complement Med. 2004;10(2):369- 78. doi: 10.1089/107555304323062365, PMID 15165418
32. Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia. 2008;51(2):216-26. doi: 10.1007/s00125-007- 0886-7, PMID 18087688
33. Gupta RK, Kesari AN, Murthy PS, Chandra R, Tandon V, Watal G. Hypoglycemic and antioxidant effects of ethanolic extract of Momordica charantia in experimental diabetes. Int J Pharm Pharm Sci. 2012;4(2):403-7.
34. Joseph B, Jini D. Antidiabetic effects of Momordica charantia (bitter melon). J Ethnopharmacol. 2013;150(2):437-48.
35. Jamadagni PS, Pawar SD, Jamadagni SB, Gautam M, Gaidhani SN, Prasad GP. Recent updates in research on Gymnema sylvestre. Pharmacogn Rev. 2021;15(30):128-33. doi: 10.5530/phrev.2021.15.15
36. Rauter AP, Martins A, Borges C, Mota-Filipe H, Pinto R, Sepodes B. Antidiabetic and antioxidant properties of plant extracts. Phytochemistry. 2010;71:1625-31.
37. Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol. 2009;41(1):40-59. doi: 10.1016/j. biocel.2008.06.010, PMID 1866280038. Patel DK, Kumar R, Laloo D, Hemalatha S. Diabetes mellitus: An overview on its pharmacological aspects and reported medicinal plants having antidiabetic activity. Int J Appl Pharm. 2018;10(3):1-9.
39. Samaddar S, Koneri R, Varre JV, Jha DK. Triterpenoid saponin from Momordica tuberosa (Cucurbitaceae) stimulates insulin secretion from isolated mouse pancreatic islets and provides protectionfrom streptozotocin and highglucose induced injury. Ind J Pharm Educ Res. 2019;53(3):511-20. doi: 10.5530/ijper.53.3.83
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