PROANTHOCYANIDIN-PLGA NANOPARTICLE INFUSED CALCIUM HYDROXIDE SEALER: ADVANCING MATERIAL PERFORMANCE AND CLINICAL STABILITY IN ENDODONTICS

VANDANA SADANANDA; GOWRISH S.; R. NARAYANA CHARYULU; MITHRA N. HEGDE

doi:10.22159/ijap.2025v17i2.53094

Authors

VANDANA SADANANDA Department of Conservative Dentistry and Endodontics, Deralakatte, Mangaluru-575018, Karnataka, India, https://orcid.org/0000-0003-1546-4978
GOWRISH S. Department of Conservative Dentistry and Endodontics, Deralakatte, Mangaluru-575018, Karnataka, India https://orcid.org/0000-0002-0600-7794
R. NARAYANA CHARYULU NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Deralakatte, Mangaluru-575018, Karnataka, India https://orcid.org/0000-0002-4404-0296
MITHRA N. HEGDE Department of Conservative Dentistry and Endodontics, Deralakatte, Mangaluru-575018, Karnataka, India https://orcid.org/0000-0002-5618-6778

DOI:

https://doi.org/10.22159/ijap.2025v17i2.53094

Keywords:

Calcium hydroxide, Proanthocyanidin nanoparticles, PLGA, Dentine collagen stabilization

Abstract

Objective: The study presents a novel calcium hydroxide-based endodontic sealer infused with proanthocyanidin nanoparticles (PAC-NPs) encapsulated in poly(actic-co-glycolic acid) (PLGA), designed to enhance critical clinical properties and overcome limitations of traditional sealers.

Methods: The sealer's formulation was evaluated for flow, setting time, solubility, dimensional stability, and pH, adhering to ISO 6876:2012 and ANSI/ADA standards 57. The incorporation of PAC-PLGA and calcium hydroxide nanoparticles was analyzed using Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) to confirm chemical interactions and functional integration.

Results: The sealer exhibited exceptional flow properties (23.78 mm), a balanced setting profile (initial: 65 min; final: 810 min), and low solubility (0.825%), ensuring durability and clinical reliability. Dimensional stability remained within ISO limits (0.417% over 30 days). The sealer maintained a highly alkaline environment (pH 12.3 initially, stabilizing at 10.6 over four weeks), crucial for antimicrobial activity and healing. ATR-FTIR confirmed the stable incorporation of PAC-PLGA NPs, potentially offering antioxidant and collagen-stabilizing benefits.

Conclusion: This novel formulation demonstrates significant improvements in critical clinical parameters, offering a durable and bioactive alternative to traditional calcium hydroxide sealers. While no immediate limitations were observed, further in vivo assessments are recommended to evaluate its long-term sealing efficacy, dentine preservation, and periapical healing outcomes.

References

Foreman PC, Barnes IE. Review of calcium hydroxide. Int Endod J. 1990 Nov;23(6):283-97. doi: 10.1111/j.1365-2591.1990.tb00108.x, PMID 2098345.

Mohammadi Z, Dummer PM. Properties and applications of calcium hydroxide in endodontics and dental traumatology. Int Endod J. 2011 Aug;44(8):697-730. doi: 10.1111/j.1365-2591.2011.01886.x, PMID 21535021.

Kim D, Kim E. Antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: a literature review-part I. In vitro studies. Restor Dent Endod. 2014 Nov;39(4):241-52. doi: 10.5395/rde.2014.39.4.241, PMID 25383341.

Siqueira JF Jr, Lopes HP. Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J. 1999 Sep;32(5):361-9. doi: 10.1046/j.1365-2591.1999.00275.x, PMID 10551109.

Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM. Collagen degradation by host-derived enzymes during aging. J Dent Res. 2004;83(3):216-21. doi: 10.1177/154405910408300306, PMID 14981122.

Siqueira JF Jr, Rocas IN, Ricucci D, Hulsmann M. Causes and management of post-treatment apical periodontitis. Br Dent J. 2014;216(6):305-12. doi: 10.1038/sj.bdj.2014.200, PMID 24651336.

Chopra V, Davis G, Baysan A. Physico-chemical properties of calcium-silicate vs. resin based sealers-a systematic review and meta-analysis of laboratory-based studies. Materials (Basel). 2021;15(1):229. doi: 10.3390/ma15010229, PMID 35009375.

Capuano N, Amato A, Dell’Annunziata F, Giordano F, Folliero V, Di Spirito F. Nanoparticles and their antibacterial application in endodontics. Antibiotics (Basel). 2023;12(12):1690. doi: 10.3390/antibiotics12121690, PMID 38136724.

Javidi M, Zarei M, Naghavi N, Mortazavi M, Nejat AH. Zinc oxide nano-particles as sealer in endodontics and its sealing ability. Contemp Clin Dent. 2014;5(1):20-4. doi: 10.4103/0976-237X.128656, PMID 24808690.

Sabatini C, Pashley DH. Mechanisms regulating the degradation of dentin matrices by endogenous dentin proteases and their role in dental adhesion. A review. Am J Dent. 2014 Aug;27(4):203-14. PMID 25831604.

Aguiar TR, Vidal CM, Phansalkar RS, Todorova I, Napolitano JG, McAlpine JB. Dentin biomodification potential depends on polyphenol source. J Dent Res. 2014 Apr;93(4):417-22. doi: 10.1177/0022034514523783, PMID 24574140.

Bedran Russo AK, Pauli GF, Chen SN, McAlpine J, Castellan CS, Phansalkar RS. Dentin biomodification: strategies, renewable resources and clinical applications. Dent Mater. 2014 Jan;30(1):62-76. doi: 10.1016/j.dental.2013.10.012, PMID 24309436.

Makadia HK, Siegel SJ. Poly lactic-co-glycolic acid (PLGA) as biodegradable controlled drug delivery carrier. Polymers (Basel). 2011 Sep 1;3(3):1377-97. doi: 10.3390/polym3031377, PMID 22577513.

Shariati A, Chegini Z, Ghaznavi-Rad E, Zare EN, Hosseini SM. PLGA-based nanoplatforms in drug delivery for inhibition and destruction of microbial biofilm. Front Cell Infect Microbiol. 2022 Jun 21;12:926363. doi: 10.3389/fcimb.2022.926363, PMID 35800390.

Cao L, Xie X, Yu W, Xu HH, Bai Y, Zhang K. Novel protein-repellent and antibacterial polymethyl methacrylate dental resin in water-aging for 6 mo. BMC Oral Health. 2022 Oct 29;22(1):457. doi: 10.1186/s12903-022-02506-6, PMID 36309721.

Han B, Jaurequi J, Tang BW, Nimni ME. Proanthocyanidin: a natural crosslinking reagent for stabilizing collagen matrices. J Biomed Mater Res A. 2003;65(1):118-24. doi: 10.1002/jbm.a.10460, PMID 12635161.

Castellan CS, Pereira PN, Grande RH, Bedran Russo AK. Mechanical characterization of proanthocyanidin-dentin matrix interaction. Dent Mater. 2010;26(10):968-73. doi: 10.1016/j.dental.2010.06.001, PMID 20650510.

Liu R, Fang M, Xiao Y, Li F, Yu L, Zhao S. The effect of transient proanthocyanidins preconditioning on the cross-linking and mechanical properties of demineralized dentin. J Mater Sci Mater Med. 2011 Nov;22(11):2403-11. doi: 10.1007/s10856-011-4430-4, PMID 21979164.

Mazzoni A, Angeloni V, Apolonio FM, Scotti N, Tjäderhane L, Tezvergil Mutluay A. Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems. Dent Mater. 2013;29(10):1040-7. doi: 10.1016/j.dental.2013.07.010, PMID 23916318.

Xu HH, Weir MD, Sun L, Takagi S, Chow LC. Effects of calcium phosphate nanoparticles on Ca-PO4 composite. J Dent Res. 2007;86(4):378-83. doi: 10.1177/154405910708600415, PMID 17384036.