HISTOLOGICAL ANALYSIS OF HEPATOPROTECTIVE EFFECTS OF EUGENIA UNIFLORA L. LEAF ETHANOLIC EXTRACT IN BALB/C MICE
DOI:
https://doi.org/10.22159/ijap.2025.v17s1.06Keywords:
Eugenia uniflora L, Ethanolic extract, Histopathology, Hepatoprotective, Carbon tetrachlorideAbstract
Objective: The plant Eugenia uniflora L. contains various bioactive compounds, including flavonoids, polyphenols, and terpenoids. These compounds can neutralize free radicals and reduce oxidative damage and inflammation in liver tissues, thereby potentially protecting the liver from damage caused by oxidative stress. The purpose of this study is to assess the effect of Eugenia uniflora L. Leaf Ethanolic Extract (EULEE) administration on the histopathological profile in male mice induced by carbon tetrachloride (CCl4).
Methods: A total of 45 mice were assigned into 5 treatment groups, with 9 mice in each group. The negative control group was orally treated with Na-CMC suspension. The positive control group was treated orally with a 10% CCl4 solution. The treatment groups were administered a 10% CCl4 solution orally to induce hepatotoxicity, along with EULEE at doses of 200 mg/kg, 400 mg/kg, and 800 mg/kg BW. EULEE was administered for 7, 14, and 21 d. On days 8, 15, and 22, three mice from each treatment group were euthanized, and their liver organs were collected for histology analysis followed the Manja Roenigk Histopathology Scoring System. Statistical analysis was performed using two-way ANOVA.
Results: The administration of EULEE 200, EULEE 400, EULEE 800 resulted in a reduction of liver histology scores compared to the positive control group, indicating a decrease in the level of liver tissue damage. The average histology scores for the three dosage groups were 39.556±0.0555, 47.600±0.555, and 45.333±0.555.
Conclusion: The administration of EULEE results in an improvement in the liver's histological features in mice.
References
Alshehri M, Wahab Amjad M, Mudawi ME. Drugs-inducing hepatotoxicity. Asian J Pharm Res Heal Care. 2020;12(3):148-56. doi: 10.18311/ajprhc/2020/25518.
Lee GH, Lee HY, Choi MK, Chung HW, Kim SW, Chae HJ. Protective effect of Curcuma longa L. extract on CCl4 induced acute hepatic stress. BMC Res Notes. 2017;10(1):77. doi: 10.1186/s13104-017-2409-z, PMID 28143589.
Sinoriya SK, Singh K. Biochemical and histological study of three different multiherbal compositions on hepatoxicity induced by carbon tetra chloride (CCl4) in albino rats. Int J Pharm Pharm Sci. 2024;16(10):5-14. doi: 10.22159/ijpps.2024v16i10.51203.
Adewale OB, Adekeye AO, Akintayo CO, Onikanni A, Saheed S. Carbon tetrachloride (CCl4) induced hepatic damage in experimental sprague dawley rats: antioxidant potential of Xylopia aethiopica. J Phytopharmacol. 2014;3(2):118-23. doi: 10.31254/phyto.2014.3207.
Hadi AA, Alwan SF. Histopathological changes in gills, liver and kidney of freshwater fish, Tilapia zillii, exposed to aluminum. Int J Pharm Lif Sci. 2012;3(11):2071-81.
Josino Soares D, Walker J, Pignitter M, Walker JM, Imboeck JM, Ehrnhoefer Ressler MM. Pitanga (Eugenia uniflora L.) fruit juice and two major constituents thereof exhibit anti-inflammatory properties in human gingival and oral gum epithelial cells. Food Funct. 2014 Nov;5(11):2981-8. doi: 10.1039/c4fo00509k, PMID 25228206.
Kasdallah Grissa A, Mornagui B, Aouani E, Hammami M, El May M, Gharbi N. Resveratrol, a red wine polyphenol, attenuates ethanol-induced oxidative stress in rat liver. Life Sci. 2007;80(11):1033-9. doi: 10.1016/j.lfs.2006.11.044, PMID 17258234.
Rattmann YD, De Souza LM, Malquevicz Paiva SM, Dartora N, Sassaki GL, Gorin PA. Analysis of flavonoids from Eugenia uniflora leaves and its protective effect against murine sepsis. Evid Based Complement Alternat Med. 2012;2012:623940. doi: 10.1155/2012/623940, PMID 23320032.
Yao H, Qiao YJ, Zhao YL, Tao XF, Xu LN, Yin LH. Herbal medicines and nonalcoholic fatty liver disease. World J Gastroenterol. 2016;22(30):6890-905. doi: 10.3748/wjg.v22.i30.6890, PMID 27570425.
Abirami A, Nagarani G, Siddhuraju P. In vitro antioxidant, anti-diabetic, cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrix and C. maxima fruits. Food Sci Hum Wellness. 2014;3(1):16-25. doi: 10.1016/j.fshw.2014.02.001.
Dey A, Lakshmanan J. The role of antioxidants and other agents in alleviating hyperglycemia-mediated oxidative stress and injury in liver. Food Funct. 2013;4(8):1148-84. doi: 10.1039/c3fo30317a, PMID 23760593.
Daniel GS, Krishnakumar I. Quantitative analysis of primary and secondary metabolities in an aqueous hot extract of Eugenia uniflora l leaves. J Pharmacogn Phytochem. 2015;4(2):40-3.
Amorim AC, Lima CK, Hovell AM, Miranda AL, Rezende CM. Antinociceptive and hypothermic evaluation of the leaf essential oil and isolated terpenoids from Eugenia uniflora L. (Brazilian Pitanga). Phytomedicine. 2009;16(10):923-8. doi: 10.1016/j.phymed.2009.03.009, PMID 19423309.
Fiuza TS, Sabio Morais SM, Paulo JR. Evaluation of the antimicrobial activity of the crude ethanol extract of Eugenia uniflora L. leaves. J Appl Pharm Sci. 2009;29(3):245-50.
Jain A, Jain S, Kothari N. An experimental study performed to compare the hepatoprotective activity of Aloe vera and silymarin in carbon tetra chloride (CCl4)-induced hepatotoxicity in albino rabbits. Asian J Pharm Clin Res. 2022;15(4):90-4. doi: 10.22159/ajpcr.2022.v15i4.44570.
Bagetti M, Facco EM, Piccolo J, Hirsch GE, Rodriguez Amaya D, Kobori CN. Physicochemical characterization and antioxidant capacity of pitanga fruits (Eugenia uniflora L.). Cienc Tecnol Aliment. 2011;31(1):147-54. doi: 10.1590/S0101-20612011000100021.
Denardin CC, Parisi MM, Martins LA, Terra SR, Borojevic R, Vizzotto M. Antiproliferative and cytotoxic effects of purple pitanga (Eugenia uniflora L.) extract on activated hepatic stellate cells. Cell Biochem Funct. 2014;32(1):16-23. doi: 10.1002/cbf.2965, PMID 23475531.
de Melo Candeia GL, Costa WK, de Oliveira AM, Napoleão TH, Guedes Paiva PM, Ferreira MR. Anti-inflammatory, antinociceptive effects and involvement of opioid receptors in the antinociceptive activity of Eugenia uniflora leaves obtained with water, ethanol, and propylene glycol mixture. J Ethnopharmacol. 2022;296:115508. doi: 10.1016/j.jep.2022.115508, PMID 35779820.
Schumacher NS, Colomeu TC, de Figueiredo D, Carvalho C, Cazarin CB, Prado MA. Identification and antioxidant activity of the extracts of Eugenia uniflora Leaves. Characterization of the anti-inflammatory properties of aqueous extract on diabetes expression in an experimental model of spontaneous type 1 diabetes (NOD Mice). Antioxidants (Basel). 2015;4(4):662-80. doi: 10.3390/antiox4040662, PMID 26783951.
Harbone JB. A guide to modern techniques of plant analysis. 3rd ed. London: Chapman & Hall; 1988. p. 14-317.
Trefts E, Gannon M, Wasserman DH. The liver. Curr Biol. 2017;27(21):R1147-51. doi: 10.1016/j.cub.2017.09.019, PMID 29112863.
Farghali H, Canova NK, Zakhari S. Hepatoprotective properties of extensively studied medicinal plant active constituents: possible common mechanisms. Pharm Biol. 2015;53(6):781-91. doi: 10.3109/13880209.2014.950387, PMID 25489628.
Amado JR, Prada LA, Arranz EJ, Roses PR, Quevedo MH, Keita H. Antioxidant and Hepatoprotective activity of a new tablet formulation from Tamarindus indica L. Evidence-Based Complementary and Alternative Medicine. 2016;1-7.
Lee MS, Kim YH, Park WS, Ahn WG, Park OK, Kwon SH. Novel antidepressant-like activity of propolis extract mediated by enhanced glucocorticoid receptor function in the hippocampus. Evid Based Complement Alternat Med. 2013;2013:217853. doi: 10.1155/2013/217853, PMID 23853655.
Shao SY, Qi XZ, Sun H, Li S. Hepatoprotective lignans and triterpenoids from the roots of Kadsura longipedunculata. Fitoterapia. 2020;142(2):104487. doi: 10.1016/j.fitote.2020.104487, PMID 31987981.
Hadisoewignyo L, Soeliono I, Hartono SB, Hestianah EP, Mahanani SR. Hepatoprotective effects of curcumin-mesoporous silica nanoparticles on CCl 4-induced hepatotoxicity wistar rats. Indonesian J Pharm. 2019;30(2):114-21. doi: 10.14499/indonesianjpharm30iss2pp114-121.
Al-Sayed E, Martiskainen O, Seif El-Din SH, Sabra AN, Hammam OA, El-Lakkany NM. Hepatoprotective and antioxidant effect of bauhinia hookeri extracts against carbon tetrachloride-induced hepatotoxicity in mice and characterization of its bioactive compounds by HPLC-PDA-ESI-MS/MS. BioMed Res Int. 2014;2014(4):245171. doi: 10.1155/2014/245171, PMID 24955350.
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