OPTIMIZATION OF THE CONDITION TO PRODUCE ZEIN-ACALYPHA INDICA. l LEAF EXTRACT NANOPARTICLES AS ANTIOXIDANTS
DOI:
https://doi.org/10.22159/ijap.2025.v17s1.07Keywords:
Acalypha indica, Zein Nanoparticles, PSA, TEM, DPPHAbstract
Objective: Acalypha indica L. (A. indica L.) is an herbal plant predominantly found in the wet tropics and known for its diverse activities. One way to increase its biological activities is by making it as nanoparticle form. This study aimed to optimize the condition to produce zein-leaf A. indica L. Nanoparticles (NPZA) as antioxidant.
Methods: The nanoparticles condition optimization was developed using a central composite design, employing two solvents for extraction: 50% ethanol (NPZAE50%) and methanol p. a (NPZAM). The optimized conditions include the amount of extract, the amount of zein, and the sonication time. The antioxidant capacity was evaluated using the Diphenyl Picryl Hydrazyl Method, while the nanoparticles were characterized using UV/Vis Spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), Particle Size Analyzer (PSA), and Transmission Electron Microscope (TEM).
Results: The optimum condition was found on 100 mg of extract ethanol 50%, 150 mg of zein, and a sonication time of 20 min, with antioxidant capacity of 0.935±0.15 mmol AAE/ml for NPZAE50%. The average particle size of NPZAE50% was 27.5 nm with a spherical particle morphology.
Conclusion: In conclusion, the highest antioxidant capacity was achieved with 117 mg of extract, 175 mg of zein, and 23 min of sonication, with formula 20 showing the best results for NPZAE50% and NPZAM.
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Copyright (c) 2025 DINI HANIFA, FITRATUL ILAHI, YEFRIDA YEFRIDA, IRMANIDA BATUBARA, UTAMI DYAH SYAFITRI, REFILDA REFILDA
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