PHYTOCHEMICAL STUDY AND ANTIMICROBIAL PROPERTIES OF FILMY FERN (HYMENOPHYLLUM IMBRICATUM BLUME)
DOI:
https://doi.org/10.22159/ijap.2025.v17s1.16Keywords:
Filmy fern, Hymenophyllum imbricatum blume, Antimicrobial, Human pathogen bacterialAbstract
Objective: The research aimed for phytochemical study and screening antimicrobial activities of filmy fern Hymenophyllum imbricatum Blume.
Methods: Antimicrobial assay used agar diffusion method against several pathogenic bacteria: Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 14028, Salmonella typhosa NCTC 786, Bacillus subtillis ATCC 6633, Enterococcus faecalis ATCC 29212, Micrococcus luteus ATCC 10240, Salmonella typhi, Staphylococcus epidermidis ATCC 12228, Streptococcus mutans ATCC 25175, Vibrio cholera Inaba and Methicillin-Resistant Staphylococcus aureus (MRSA), the isolation used silica column chromatography, purification by recrystallization, and identification by spectroscopic techniques (NMR, MS, UV-Vis, IR).
Results: Two compounds have been isolated from the aerial part of Hymenophyllum imbricatum Blume as 4-acetylphenyl ether (1) and genkwanin-4”-O-β-glucopyranose (2). The activity inhibition of isolated compounds against some human pathogenic bacteria S. aureus ATCC 25923, E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. typhimurium ATCC 14028, S. typhosa NCTC 786, B. subtillis ATCC 6633, E. faecalis ATCC 29212, M. luteus ATCC 10240, S. typhi, S. epidermidis ATCC 12228, S. mutans ATCC 25175, V. cholera Inaba, and Methicillin-Resistant S. aureus (MRSA) was inhibited at concentrations ranging from 0.6-10 µg/disc.
Conclusion: Flavonoid glycoside and dimer compounds were isolated from Himenophyllum imbricatum; both isolated compounds showed lower inhibition in some bacteria compared to the ethyl acetate fraction, which obtained moderate inhibition against all the testing bacteria.
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