NANOEMULSION FORMULATION OF CARDAMOM ESSENTIAL OIL (AMOMUM COMPACTUM S.) AND IT’S IN VITRO ANTI-AGING ACTIVITY
DOI:
https://doi.org/10.22159/ijap.2025.v17s1.20Keywords:
Cardamom essential oil, Anti-aging, Nanoemulsion, Tyrosinase, Elastase, HyaluronidaseAbstract
Objective: Cardamom fruit (Amomum compactum S.) is one of the most famous spices in the world. Its essential oil has anti-aging activity. This study aims to formulate a stable anti-aging nanoemulsion preparation of Cardamom Essential Oil (CEO) using Tween 80 and Pluronic F127 as surfactants and co-surfactants.
Methods: Optimization began by analyzing the transmittance and pH results as affected by Tween 80 and Pluronic F127. The optimum surfactant/cosurfactant composition was used to make cardamom oil nanoemulsion serum by adding grape seed oil as the oil phase. Evaluation of nanoemulsion preparation included organoleptic, particle size, polydispersity index (PDI), zeta potential, pH, viscosity, emulsion type, and stability test by freeze and thaw cycle test and centrifugation. The most optimal and stable nanoemulsion formula was tested for its activity in inhibiting anti-aging enzymes (tyrosinase, elastase, and hyaluronidase).
Results: According to the analysis, the optimal ratio of Tween 80 and Pluronic F127 for nanoemulsion formation was 8.5 ml: 0.15 g. The nanoemulsion was pale yellow with a distinctive cardamom flavor, had a globule size of 10.8±0.5 nm with a polydispersity index (PDI) of 0.207±0.1, zeta potential of-19.33±0.5 mV, pH of 7.44±0.29, viscosity of 394 cPs, with type M/A emulsion. The nanoemulsion preparation actively inhibited tyrosinase, elastase, and hyaluronidase enzymes with IC50 2.45 µl/ml, 20 µl/ml, and 4.80 µl/ml, respectively. The preparation with the strongest anti-aging effect on tyrosinase and hyaluronidase enzymes was a mixture of amomum essential oil and grape seed oil, which remained stable after the stability test.
Conclusion: It can be concluded that the nanoemulsion formula meets the characterization of nanoemulsion preparations and is active as an anti-aging.
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