DOSE DEPENDENT IMUNOMODULATORY EFFECTS OF THE HERBAL COMBINATION SAMBILOTO–GINGER–TURMERIC (SIJAKUN): AN IN VIVO STUDY ON WISTAR RATS
DOI:
https://doi.org/10.22159/ijap.2026v18i2.56973Keywords:
SIJAKUN, Immunomodulator, Dose-dependent, Inflammation, Herbal-combination, CytokineAbstract
Objective: This study aimed to evaluate the effects of Sijakun at different doses on key inflammatory parameters-Neutrophil-to-Lymphocyte Ratio (NLR), macrophage count, TNF-α, and IL-6-in an LPS-induced inflammatory model.
Methods: An experimental study was conducted using animal subjects randomly allocated into five groups: negative control, LPS-induced positive control, and three treatment groups receiving Sijakun at graded doses. Inflammatory markers were assessed post-intervention. Data were analyzed using ANOVA for normally distributed parameters and Kruskal–Wallis for non-normal data, followed by appropriate post hoc tests.
Results: Sijakun administration significantly influenced all measured parameters (p<0.05). The highest dose (500 mg/kg BW) most effectively reduced NLR, TNF-α, and IL-6 levels, bringing them close to baseline control values. In contrast, the medium dose (250 mg/kg BW) optimally increased macrophage count, indicating enhanced innate immune response.
Conclusion: Sijakun exerts significant anti-inflammatory and immunomodulatory effects in a dose-dependent manner. A high dose (500 mg/kg BW) is most effective in suppressing systemic and cytokine-mediated inflammation, while a medium dose (250 mg/kg BW) best enhances macrophage-mediated phagocytosis. These findings support the potential of Sijakun as a complementary herbal agent for modulating LPS-induced inflammatory responses.
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Copyright (c) 2026 HANDAYANI, HOTIMAH MASDAN SALIM, ARIFA MUSTIKA, AIN DAROJAH SIDDIQ RAMADHANA, RETNO DIAH PUTRI EKAYANTI
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