IN VITRO CYTOTOXICITY AND WOUND HEALING EFFICACY OF BIOSYNTHESIZED SILVER NANOPARTICLES FROM AGERATINA ADENOPHORA AQUEOUS STEM EXTRACT
DOI:
https://doi.org/10.22159/ijap.2026v18i3.57193Keywords:
Silver nanoparticles, Antimicrobial, Antioxidant, Cytotoxicity, Wound healingAbstract
Objective: The present study was aimed to synthesize biogenic silver nanoparticles (AgNPs) from Ageratina adenophora (A. adenophora) aqueous stem extract and also to study its antimicrobial, antioxidant, cytotoxic and enhanced wound healing properties on Vero cell line.
Methods: The biogenic AgNPs were synthesized using aqueous stem extracts of A. adenophora and were characterized using ultraviolet - visible spectroscopy, High-resolution transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR). The AgNPs were tested for their antibacterial and antifungal efficacy using the agar well diffusion method. The DPPH scavenging ability of AgNPs was studied, and MTT assay was used to evaluate the cytotoxic effects and wound scratch assay was performed to study the potential healing activity of biosynthesized AgNPs on Vero cells.
Results: AgNPs synthesized using A. adenophora aqueous stem extract showed good stability, with maximum absorption spectra of 450 nm. The synthesized AgNPs were found to be spherical in shape with a size range between 20 - 60 nm. The XRD indicates that the crystalline structure of AgNPs is a face-centred cubic (fcc) crystal. The AgNPs showed good stability and scavenging activity. The Vero cell line at lower concentrations display acceptable biocompatibility for AgNPs with IC50 concentration of 108.61 µg/mL, indicating concentration-dependent cytotoxicity in vitro. The synthesised AgNPs demonstrated 57.97 % wound closure rate, indicating substantial potential for wound healing.
Conclusion: According to the results, the synthesized AgNPs using aqueous stem extract of A. adenophora has demonstrates effective wound healing potential on Vero cell lines.
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