EVALUATION OF ANTI-DEPRESSANT ACTIVITY OF ETHANOLIC EXTRACT OF PIPER METHYSTICUM LEAVES IN MICE
DOI:
https://doi.org/10.22159/ijcpr.2026v18i2.8017Keywords:
Antidepressant, Fluoxetine, Piper methysticum, Oxidative stress, Major depressive disorderAbstract
Objective: The aim of this study to evaluate the anti-depressant activity of ethanolic extract of Piper methysticum leaves in mice.
Methods: The animals were grouped into four groups, each comprising 6 mice. One of the group as control, receiving normal saline water and second group as standard, receiving fluoxetine, while the remaining two groups were administered the test drug (300 mg/kg and 600 mg/kg). All drug administrations were conducted orally 1 h prior to the acute study test procedure and daily for a 14 d period for the chronic study.
Results: The administration of Piper methysticum doses resulted in a notable reduction in immobility duration during the Tail Suspension Test. In the Actophotometer assessment, mice exhibited a statistically significant increase in motor activity compared to the control group. The findings indicated that the ethanolic extract of Piper methysticum leaves at a dose of 600 mg/kg led to a significant increase in reduced glutathione and catalase activity. Simultaneously, there was a decrease in plasma corticosterone, lipid peroxidation, plasma nitrite, and protein concentration levels.
Conclusion: The antidepressant efficacy of Piper methysticum at a dosage of 600 mg/kg in mice is comparable to that of fluoxetine.
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