EVALUATION OF DIURETIC ACTIVITY OF ALCOHOLIC EXTRACT OF FLOWER BUDS OF NEOLAMARCKIA CADAMBA IN ALBINO RATS
DOI:
https://doi.org/10.22159/ijcpr.2026v18i2.8018Keywords:
Flower buds of Neolamarckia cadamba, Alcoholic extract, Hydrated rats, Diuretic activity, Metabolic cageAbstract
Objective: To study the diuretic activity of Alcoholic Extract of Flower buds of Neolamarckia cadamba by Lipschitz method in albino Wistar rats.
Methods: Metabolic cages were utilised to test the diuretic effect of Alcoholic Extract of Flower Buds of Neolamarckia cadamba in four Albino Wistar rats. Group I, the normal control, got vehicle (10 ml/kg or 1 ml of 0.9% NaCl/100 gm), group II received Frusemide (10 mg/Kg, p. o), and groups III and IV received low (200 mg/kg) and high (400 mg/kg) dosages of Alcoholic Extract of Neolamarckia cadamba flower buds Following Neolamarckia cadamba Alcoholic Extract treatment, rats were hydrated with saline (15 ml/kg, p. o) and put in metabolic cages at 25 °C±0.5 °C. Animals went 5 h without food or water. Each metabolic cage's urine volume was assessed after 5 and 24 h. Total urine volume, ion concentration (Sodium, Potassium, Chloride), and pH were measured.
Results: In this model when compared to control group the Alcoholic Extract of Flower buds of Neolamarckia cadamba treated groups at different dose levels (200 and 400 mg/kg) have noted with significant increase in the urine volume and also significantly enhanced the excretion of Sodium, Potassium and Chloride ions in urine. In the urine pH no difference has been found.
Conclusion: Results showed that single dose administration of standard Furosemide and Alcoholic Extract of Flower buds of Neolamarckia cadamba significantly increased the urine output along with an increase in elimination of Sodium, Potassium, and Chloride ions. Alcoholic Extract of Flower buds of Neolamarckia cadamba, 400 mg/Kg produced a comparable diuretic activity with standard Frusemide.
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References
1. Dehkordi FR, Kamkhah AF. Antihypertensive effect of Nigella sativa seed extract in patients with mild hypertension. Fundam Clin Pharmacol. 2008;22(4):447-52. doi: 10.1111/j.1472-8206.2008.00607.x, PMID 18705755.
2. Hobson RM, Maughan RJ. Hydration status and the diuretic action of a small dose of alcohol. Alcohol Alcohol. 2010;45(4):366-73. doi: 10.1093/alcalc/agq029, PMID 20497950.
3. Jimenez Ferrer E, Alarcon Alonso J, Aguilar Rojas A, Zamilpa A, Jimenez Ferrer C I, Tortoriello J. Diuretic effect of compounds from Hibiscus sabdariffa by modulation of the aldosterone activity. Planta Med. 2012;78(18):1893-8. doi: 10.1055/s-0032-1327864, PMID 23150077.
4. Kreydiyyeh SI, Usta J. Diuretic effect and mechanism of action of parsley. J Ethnopharmacol. 2002;79(3):353-7. doi: 10.1016/S0378-8741(01)00408-1, PMID 11849841.
5. Leong XF, Rais Mustafa MR, Jaarin K. Nigella sativa and its protective role in oxidative stress and hypertension. Evid Based Complement Alternat Med. 2013;2013:120732. doi: 10.1155/2013/120732, PMID 23533459.
6. Zeratsky K. Weight loss can natural diuretics reduce fluid retention and help with weight loss? Mayo Clinic Publications. 2015 Mar 4. Available from: http://www.mayoclinic.org/healthy-lifestyle/weight-loss/expert-answers/water-retention/faq-20058063. [Last accessed on 31 Jan 2026].
7. DaSilva EJ, Baydoun E, Badran A. Biotechnology and the developing world. Electron J Biotechnol. 2002;5(1):5-6. doi: 10.2225/vol5-issue1-fulltext-1.
8. Cravotto G, Boffa L, Genzini L, Garella D. Phytotherapeutics: an evaluation of the potential of 1000 plants. J Clin Pharm Ther. 2010;35(1):11-48. doi: 10.1111/j.1365-2710.2009.01096.x, PMID 20175810.
9. Gilani AH, Shah AJ, Zubair A, Khalid S, Kiani J, Ahmed A. Chemical composition and mechanisms underlying the spasmolytic and bronchodilatory properties of the essential oil of Nepeta cataria L. J Ethnopharmacol. 2009;121(3):405-11. doi: 10.1016/j.jep.2008.11.004, PMID 19041706.
10. Talalay P, Talalay P. The importance of using scientific principles in the development of medicinal agents from plants. Acad Med. 2001;76(3):238-47. doi: 10.1097/00001888-200103000-00010, PMID 11242573.
11. Elvin Lewis M. Should we be concerned about herbal remedies? J Ethnopharmacol. 2001;75(2-3):141-64. doi: 10.1016/S0378-8741(00)00394-9, PMID 11297844.
12. Muller JL. Love potions and the ointment of witches: historical aspects of the nightshade alkaloids. J Toxicol Clin Toxicol. 1998;36(6):617-27. doi: 10.3109/15563659809028060, PMID 9776969.
13. Dwevedi A, Sharma K, Sharma YK. Cadamba: a miraculous tree having enormous pharmacological implications. Pharmacogn Rev. 2015;9(18):107-13. doi: 10.4103/0973-7847.162110, PMID 26392707.
14. Banerji N, Siddhanta AK, Dutta NL. New saponin from stem bark of Anthocephalus cadamba Miq. Indian J Chem B. 1977;15:654-5.
15. Nadkarni KM. Indian materia medica. Bombay: Popular Press Prakashan; 2002. p. 118-9.
16. Kirtikar KR, Basu BD. Indian medicinal plants. 2nd ed. Allahabad: Lalit Mohan Basu Publishers; 1999. p. 1250-2.
17. Kapil A, Koul IB, Suri OP. Antihepatotoxic effects of chlorogenic acid from Anthocephalus cadamba. Phytother Res. 1995;9(3):189-93. doi: 10.1002/ptr.2650090307.
18. Nadkarni KM. Indian materia medica. 3rd ed. Bombay: Popular Press Prakashan; 1999. p. 118-9.
19. Sahu NP, Chakraborty B, Banerjee S. A new triterpenic acid from Anthocephalus cadamba Miq. Indian J Pharm Sci. 1974;12:284-6.
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