PRELIMINARY PHARMACOLOGICAL SCREENING OF MICROCOCCA MERCURIALI (L.) BENTH. LEAVES EXTRACTS FOR WOUND HEALING ACTIVITY
DOI:
https://doi.org/10.22159/ijcpr.2026v18i1.8052Keywords:
Wound healing, Micrococcamercurialis, Hydroxyproline, Povidone-iodine ointment, FlavonoidsAbstract
Objective: Wound healing is a complex physiological process that requires a series of steps, each with several factors to come to completion. The objective of present study was to screen different extracts of Micrococca mercurialis (L.) Benth. leaves for wound healing activity using incision and excision wound model in experimental animals.
Methods: Different extracts of Micrococca mercurialis leaves were obtained by extraction with petroleum ether, chloroform, acetone, ethyl acetate, methanol and water. All these extracts were tested for detection of phytochemical constituents using different chemical test qualitatively. Preliminary wound healing activity was tested using incision and excision wound models and observed tensile strength of wound tissue in incision wound model. Wound contraction and biochemical estimation from wound tissue were observed in excision wound model.
Results: Results of present study was confirmed that methanol extract of Micrococca mercurialis leaves was showed higher tensile strength significantly (P<0.05) in comparison to the control group of animals. Hydroxyproline content and protein content were found significant (P<0.05) greater than the other extracts and standard marketed formulation (Povidone-Iodine ointment).
Conclusion: In conclusion, the potent wound healing effect of Micrococca mercurialis leaves extract may be attributed by the presence of phenolic and flavonoids components in the extract. Further detail study is required to explore the mechanism behind the healing effect and specific component responsible for activity.
Downloads
References
1. Schultz GS, Chin GA, Moldawer L, Diegelmann RF. Principles of wound healing. In: Mechanisms of vascular disease: a reference book for vascular specialists. Adelaide: University of Adelaide Press; 2011. p. 423-50. doi: 10.1017/UPO9781922064004.024.
2. Shukla VK, Ansari MA, Gupta SK. Wound healing research: a perspective from India. Int J Low Extrem Wounds. 2005;4(1):7 8. doi: 10.1177/1534734604273660, PMID 15860447.
3. Chopra RN, Nayer SL, Chopra IC. Glossary of Indian medicinal plants. New Delhi: Council of Scientific and Industrial Research; 1956. p. 162.
4. Choudary S, Chowdary HR, Mandal S. Microccamercurialis benth pharmacognostic analysis and antimicrobial activity of a folk medicinal plant. J Biol Agric Healthc. 2014;4(27):122-8.
5. Kirtikar KR, Basu BD. Indian medicinal plants. Periodical expert book agency: Delhi; 1987.
6. Kokate CK, Purohit AP, Gokhale SB. Textbook of pharmacognosy. Pune: Nirali Prakasan; 2002. p. 10-8.
7. Hemalata S, Subramanian N, Ravichandran V, Chinnaswamy K. Wound healing activity of Indigoferaennaphylla linn. Indian J Pharm Sci. 2001;63(4):331-3.
8. Rashed AN, Afifi FU, Disi AM. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI‑1. J Ethnopharmacol. 2003;88(2‑3):131‑6. doi: 10.1016/S0378‑8741(03)00194‑6, PMID 12963132.
9. Kuwano H, Yano K, Ohno S, Ikebe M, Kitamura K, Toh Y. Dipyridamole inhibits early wound healing in rat skin incisions. J Surg Res. 1994;56(3):267-70. doi: 10.1006/jsre.1994.1042, PMID 8145544.
10. Taranalli AD, Tipare SV, Kumar S, Torgal SS. Wound healing activity of Oxalis corniculata whole plant extract in rats. Ind J Pharm Sci. 2004;66(4):444-6.
11. Woessner JF. The determination of hydroxyproline in tissue and protein samples containing small portion of this imino acid. Arch Biochem Biophys. 1961;93(2):440‑7. doi: 10.1016/0003‑9861(61)90291‑0.
12. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem. 1951;193(1):265-75. doi: 10.1016/S0021-9258(19)52451-6, PMID 14907713.
13. Thendral Hepsibha B, Gayathri Devi R, Lijitha L. In vitro screening of antioxidant free radical scavenging and antimicrobial potential of Micrococca mercurialiswhole plant extracts. Int J Res Pharm Sci. 2019;10(4):3251-62. doi: 10.26452/ijrps.v10i4.1630.
14. Lodhi S, Jain A, Jain AP, Pawar RS, Singhai AK. Effects of flavonoids from Martynia annua and Tephrosia purpurea on cutaneous wound healing. Avicenna J Phytomed. 2016;6(5):578-91. PMID 27761428.
15. Raghow R. The role of extracellular matrix in postinflammatory wound healing and fibrosis. FASEB J. 1994;8(11):823-31. doi: 10.1096/fasebj.8.11.8070631, PMID 8070631.
16. Sai Prasanna CG, Poongani M, Karpagam S. Phytochemical content of the leaf stem and root of Micrococca mercurialis(L.) Benth. a promising herb. IOSR J Pharm Biol Sci. 2015;10(3):24‑7.
Published
How to Cite
Issue
Section
This work is licensed under a Creative Commons Attribution 4.0 International License.