NEPHROPROTECTIVE ACTIVITY OF AERIAL PARTS OF URARIA PICTA AGAINST DRUG-INDUCED NEPHROTOXICITY IN RATS
DOI:
https://doi.org/10.22159/ijhs.2025v13.ijhs.57619Keywords:
Uraria picta, Nephroprotective activity, Gentamicin, Drug-induced nephrotoxicity, Antioxidant, Renal protection, Flavonoids, Phenolic compoundsAbstract
Objectives: The present study aimed to investigate the nephroprotective potential of the ethanolic extract of the aerial parts of Uraria picta against gentamicin-induced nephrotoxicity in Wistar rats. Additionally, the study evaluated the phytochemical composition, total phenolic content (TPC), and total flavonoid content (TFC) of the extract to understand its role in renal protection.
Methods: Preliminary phytochemical screening was conducted, followed by estimation of TPC and TFC. Nephrotoxicity was induced in rats using gentamicin (100 mg/kg, i.p.) for 8 consecutive days. The extract was administered orally at doses of 100 mg/kg and 200 mg/kg. Renal function was evaluated by estimating serum creatinine, blood urea nitrogen (BUN), urea, total protein, and albumin levels.
Results: The aerial parts of U. picta were extracted using ethanol, yielding 9.25% (w/w). Phytochemical analysis confirmed the presence of alkaloids, flavonoids, phenols, saponins, and proteins. The TPC and TFC were found to be 0.578 mg/100 mg and 0.624 mg/100 mg, respectively. Gentamicin administration caused a significant elevation in serum creatinine, BUN, and urea, along with a reduction in total protein and albumin levels, confirming renal damage. Treatment with U. picta extract resulted in a dose-dependent improvement in all biochemical parameters, with the 200 mg/kg dose showing highly significant (p < 0.001) protection, restoring values toward normal.
Conclusion: The ethanolic extract of Uraria picta demonstrated significant nephroprotective activity against gentamicin-induced renal toxicity in rats. The protective effects may be attributed to its antioxidant, anti-inflammatory, and membrane-stabilizing properties linked to its phytochemical constituents. These findings support the therapeutic potential of U. picta in managing drug-induced nephrotoxicity.
References
1. Rahman A, Penumallu NR, Abass S, Parveen B, Parveen R, Ahmad S, et al. Drug-induced nephrotoxicity and its reversal using botanicals of traditional Indian medicine in different animal models: Way forward. Future J Pharm Sci 2025;11:141.
2. Perse M. Cisplatin mouse models: Treatment, toxicity and translatability. Biomedicines 2021;9:1406.
3. Chhipa AS, Baksi R, Nivsarkar M. Anticancer studies on methanolic extract of aerial parts of Uraria picta (Jacq.) DC. Future J Pharm Sci 2021;7:16.
4. Saxena HO, Soni A, Mohammad N, Choubey SK. Phytochemical screening and elemental analysis in different plant parts of Uraria picta Desv.: A Dashmul species. J Chem Pharm Res 2014;6: 756-60.
5. Tienda-Vázquez MA, Morreeuw ZP, Sosa-Hernández JE, Cardador-Martínez A, Sabath E, Melchor-Martínez EM, et al. Nephroprotective plants: Review and mechanisms. Pharmacogn Rev 2022;11:818.
6. Mukherjee PK. Quality Control of Herbal Drugs. 2nd ed. New Delhi: Business Horizons; 2007. p. 2-14.
7. Khandelwal KR. Practical Pharmacognosy. 11th ed. New Delhi: Nirali Prakashan; 2004. p. 184.
8. Parkhe G, Bharti D. In vitro antioxidant activity, total phenolic and flavonoid contents of hydroalcoholic extract of leaves of Lagerstroemia parviflora Roxb. J Drug Deliv Ther 2019;9:708-11.
9. Bencheikh N, Ouahhoud S, Cordero MA, Alotaibi A, Fakchich J, Ouassou H, et al. Nephroprotective and antioxidant effects of flavonoid-rich extract of Thymelaea microphylla Coss. et Dur aerial part. Appl Sci 2022;12:9272.
10. Parrish AR, Chen G, Burghardt RC, Watanabe T, Morisseau C, Hammock BD. Attenuation of cisplatin nephrotoxicity by inhibition of soluble epoxide hydrolase. Cell Biol Toxicol 2008;25: 217-25.
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