A STUDY INVESTIGATING THE PRESENCE OF BIOACTIVE COMPOUNDS BY THIN-LAYER CHROMATOGRAPHY IN AVICENNIA MARINA (FORSSK.) VIERH AND THE ANTIBACTERIAL ACTIVITY OF PLANT EXTRACTS USING VARIOUS SOLVENTS

ASHA SELVA MALAR M; REENA A

doi:10.22159/ijms.2025v13i2.53614

Authors

ASHA SELVA MALAR M Department of Microbiology, Mohamed Sathak College of Arts and Science, Chennai, Tamil Nadu, India https://orcid.org/0000-0002-7128-0447
REENA A Department of Microbiology, Mohamed Sathak College of Arts and Science, Chennai, Tamil Nadu, India

DOI:

https://doi.org/10.22159/ijms.2025v13i2.53614

Keywords:

Avicennia marina (Forssk.) Vierh, Thin layer chromatography, Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19115, Antibacterial activity

Abstract

Objective: The present study aims to find out the presence of potential bioactive compounds in the mangrove plant Avicennia marina (Forssk.) Vierh, through thin-layer chromatography (TLC), observes the antibacterial activity of various solvent extracts of the plant parts against the pathogens Escherichia coli ATCC 25922 and Listeria monocytogenes ATCC 19115.

Methods: TLC of the methanol, ethyl acetate, chloroform solvent extracts of stem, root, and leaf of mangrove plant A. marina (Forssk.) Vierh was studied with the mobile phase hexane: ethyl acetate in the ratio of 1;1, 3;7, and 7;3. The spots were identified at ultraviolet 240 nm. Antibacterial activity of the solvent extracts of different plant parts was done against L. monocytogenes ATCC 19115 and E. coli ATCC 25922 by well diffusion method.

Results: The thin-layer chromatographic technique revealed the presence of the potential bioactive compound in the A. marina (Forssk.) Vierh. The solvent extracts of different plant parts showed the zone of inhibition for the antibacterial effect ranges between 1 mm and 9 mm. The methanol root extract showed a maximum inhibitory effect against L. monocytogenes ATCC 19115. The methanol stem and leaf extract showed maximum inhibition against E. coli ATCC 25922.

Conclusion: The present study emphasizes the importance of exploring the mangrove plant A. marina (Forssk.) Vierh is a potent source of bioactive compounds.

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