BIOCARRIERS AMPLIFYING ANTI-AGING: CARNOSINE LOADED NANOGEL ON D-GALACTOSE INDUCED SKIN AGING IN RAT MODEL

GOWTHAM SUNDARRAJAN; ARIHARASIVAKUMAR G.; PRIYANKA S.

doi:10.22159/ijpps.2025v17i3.53570

Authors

GOWTHAM SUNDARRAJAN Department of Pharmacology, Kmch College of Pharmacy, Tamil Nadu, India https://orcid.org/0009-0007-3771-1809
ARIHARASIVAKUMAR G. Department of Pharmacology, Kmch College of Pharmacy, Tamil Nadu, India
PRIYANKA S. Department of Pharmaceutics, Senghundhar College of Pharmacy, Tamil Nadu, India

DOI:

https://doi.org/10.22159/ijpps.2025v17i3.53570

Keywords:

Skin aging, Nanogel, Biocarriers, Carnosine peptide, Hydrosomes, Cosmeceuticals

Abstract

Objective: This study aimed to evaluate the efficacy of biocarriers in amplifying the anti-aging effects of Carnosine-Loaded Nanogel (CAR-HS) on D-Galactose (D-gal) induced skin aging in sprague dawley rats.

Methods: Thirty-four Sprague Dawley rats were divided into six groups: Group I (healthy control, no treatment), Group II (D-GAL positive control), Groups III to V (treated with carnosine-loaded gels), and Group VI (blank hydrosomes (HS_blank)). Evaluation parameters included drug content, encapsulation efficiency, vesicle size, zeta potential, polydispersity index (PDI), in vitro drug release, antioxidant markers, histopathology, and hematological analysis.

Results: Carnosine Hydrosomes (CAR-HS) demonstrated significant de-aging effects. Antioxidant marker levels (Superoxide Dismutase (SOD): 56.8±3.4 U/mg, Catalase (CAT): 85.2±4.1 U/mg, GSH: 12.6±0.8 nmol/mg, MDA: 2.1±0.2 nmol/mg) showed significant improvements (p<0.05) compared to the positive control group (SOD: 32.3±2.7 U/mg, CAT: 49.5±3.2 U/mg, Glutathione (GSH): 6.8±0.6 nmol/mg, Malondialdehyde (MDA): 4.7±0.3 nmol/mg). Histopathological analysis revealed normal epidermal structures and minimal immune cell infiltration in the hydrosomes group, while the positive control exhibited extensive damage. Hematological analysis indicated improved Red Blood Cell (RBC) (6.7±0.4 million/µl) and Hemoglobin (Hb) levels (13.5±0.7 g/dl) in the hydrosomes group compared to the positive control (RBC: 4.8±0.3 million/µl, Hb: 9.2±0.5 g/dl). Spleen histology supported these findings, showing reduced age-related changes.

Conclusion: The present study revealed that anti-aging of CAR-HS holds significant potential for tissue repair, effectively reducing oxidative stress and associated inflammation, likely through mechanisms involving cellular-level damage repair.

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