EXTRACTION AND ISOLATION OF BIOACTIVE COMPOUNDS FROM CORAL JUNCEELLA DELICATA (GRASSHOFF, 1999) FROM WEST COAST OF MUMBAI

MEENAKSHI BORATE; GAUTAM ZODAPE

doi:10.22159/ijpps.2025v17i9.54605

Authors

MEENAKSHI BORATE Department of Zoology, Chikitsak Samuha’s Sir Sitaram and Lady Shantabai Patkar College of Arts and Science and V. P. Varde College of Commerce and Economics, S. V. Road, Goregaon West, Mumbai-400104, Maharashtra, India
GAUTAM ZODAPE Department of Zoology, Chikitsak Samuha’s Sir Sitaram and Lady Shantabai Patkar College of Arts and Science and V. P. Varde College of Commerce and Economics, S. V. Road, Goregaon West, Mumbai-400104, Maharashtra, India

DOI:

https://doi.org/10.22159/ijpps.2025v17i9.54605

Keywords:

Coral, Madh island, Extraction, Bioactive compounds, Analytical techniques, Structural determination

Abstract

Objective: To isolate the bioactive compounds from coral Junceella delicata (Grasshoff, 1999) collected from West coast of Mumbai.

Methods: The coral J. delicata was collected from Patwadi Village, Madh Island, Malad West Mumbai-400061, Maharashtra, India, during the low tide. The sample was grinded with blender and then macerated by adding an equal volume of MeOH: DCM (1:1). The aliquot was concentrated in a rotary vacuum evaporator at 45 °C. The resultant compound was subjected to Millipore filter system and finally dried in vacuum desiccator. The sample was further subjected for TLC and pure compounds were processed for GC-MS and FTIR for structural determinations.

Results: The distinct and well-separated compounds were processed for GC-MS and FTIR for their structural elucidation. These compounds are (Ethyl aminomethyl formimidate); (Gly-Gly); (2-(2-Pyridyl)-4 methylthiazole-5-carboxylic acid); (7-Methoxy-2-methylquinolin-4-ol); (Fraxidin); (2-methyl-3-trans-propenylpyrazine); (3-tert-Butylpyridine); (Acetaldehyde benzyl ethyl acetal); (α-Methylcinnamic acid); (4-Ethoxycoumarin); (3-Hydroxycoumarin); (2, 4, 7, 9-Tetramethyl-5-decyne-4, 7-diol); (2,2-Bis(3-allyl-4-hydroxyphenyl) propane); (Phenyltriethylammonium cation); (Dodecanedioic acid). These compounds showed biomedical properties.

Conclusion: The isolated bioactive compounds may be used for pharmaceutical and therapeutic applications. Further studies, including molecular-level research, are necessary to confirm their mechanisms of action and clinical relevance. Additionally, safety and efficacy assessments are crucial to support the development of new pharmaceutical products aimed at improving human health.

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References

1. Zhang W, Guo YW, Gu Y. Secondary metabolites from the South China Sea invertebrates: chemistry and biological activity. Curr Med Chem. 2006;13(17):2041-90. doi: 10.2174/092986706777584960, PMID 16842196.

2. Yang F, Li SW, Zhang J, Liang LF, Lu YH, Guo YW. Uncommon nornardosinane seconeolemnane and related sesquiterpenoids from Xisha soft coral Litophyton nigrum. Bioorg Chem. 2020 Mar;96:103636. doi: 10.1016/j.bioorg.2020.103636, PMID 32045775.

3. Aratake S, Tomura T, Saitoh S, Yokokura R, Kawanishi Y, Shinjo R. Soft coral Sarcophyton (Cnidaria: Anthozoa: Octocorallia) species diversity and chemotypes. PLOS One. 2012;7(1):e30410. doi: 10.1371/journal.pone.0030410, PMID 22272344.

4. Fabricius KE, Klumpp DW. Widespread mixotrophy in reef-inhabiting soft corals: the influence of depth, and colony expansion and contraction on photosynthesis. Mar Ecol Prog Ser. 1995;125(1-3):195-204. doi: 10.3354/meps125195.

5. Van Alstyne KL, Wylie CR, Paul VJ, Meyer K. Antipredator defenses in tropical pacific soft corals (Coelenterata: Alcyonacea). I. Sclerites as defenses against generalist carnivorous fishes. Biol Bull. 1992;182(2):231-40. doi: 10.2307/1542116, PMID 29303665.

6. Dobretsov S, Al Wahaibi AS, Lai D, Al Sabahi J, Claereboudt M, Proksch P. Inhibition of bacterial fouling by soft coral natural products. Int Biodeterior Biodegrad. 2015 Mar;98:53-8. doi: 10.1016/j.ibiod.2014.10.019.

7. Sammarco PW, La Barre S, Coll JC. Defensive strategies of soft corals (Coelenterata: Octocorallia) of the Great Barrier Reef: III. The relationship between ichthyotoxicity and morphology. Oecologia. 1987;74(1):93-101. doi: 10.1007/BF00377351, PMID 28310420.

8. Ellithey MS, Lall N, Hussein AA, Meyer D. Cytotoxic and HIV-1 enzyme inhibitory activities of red sea marine organisms. BMC Complement Altern Med. 2014;14(1):77. doi: 10.1186/1472-6882-14-77, PMID 24568567.

9. Kelman D, Kushmaro A, Loya Y, Kashman Y, Benayahu Y. Antimicrobial activity of a red sea soft coral Parerythropodium fulvum fulvum: reproductive and developmental considerations. Mar Ecol Prog Ser. 1998 Aug;169:87-95. doi: 10.3354/meps169087.

10. Harder T, Lau SC, Dobretsov S, Fang TK, Qian PY. A distinctive epibiotic bacterial community on the soft coral Dendronephthya sp. and antibacterial activity of coral tissue extracts suggest a chemical mechanism against bacterial epibiosis. FEMS Microbiol Ecol. 2003;43(3):337-47. doi: 10.1111/j.1574-6941.2003.tb01074.x, PMID 19719665.

11. Gomaa MN, Soliman K, Ayesh A, Abd El Wahed A, Hamza Z, Mansour HM. Antibacterial effect of the Red Sea soft coral Sarcophyton trocheliophorum. Nat Prod Res. 2016;30(6):729-34. doi: 10.1080/14786419.2015.1040991, PMID 26186031.

12. Zhao M, Yin J, Jiang W, Ma M, Lei X, Xiang Z. Cytotoxic and antibacterial cembranoids from a South China Sea soft coral, Lobophytum sp. Mar Drugs. 2013;11(4):1162-72. doi: 10.3390/md11041162, PMID 23552878.

13. Pereira I, Severino P, Santos AC, Silva AM, Souto EB. Linalool bioactive properties and potential applicability in drug delivery systems. Colloids Surf B Biointerfaces. 2018 Nov 1;171:566-78. doi: 10.1016/j.colsurfb.2018.08.001, PMID 30098535.

14. Xu JH, Lai KH, Su YD, Chang YC, Peng BR, Backlund A. Briaviolides KN, new briarane-type diterpenoids from cultured octocoral Briareum violaceum. Mar Drugs. 2018;16(3):75. doi: 10.3390/md16030075, PMID 29495481.

15. Dyer. Applications of adsorption spectroscopy of organic compounds. Englewood Cliffs: Prentice Hall of India Private Limited; 1974.

16. Brian F, Antoni J, Hannaford P, Smith WG, Tatchell AR. Textbook of Organic Chemistry. Longman Group, UK Ltd; 1989.

17. Gonzalez Y, Doens D, Santamaria R, Ramos M, Restrepo CM, Barros De Arruda LB. A pseudopterane diterpene isolated from the octocoral Pseudopterogorgia acerosa inhibits the inflammatory response mediated by TLR-ligands and TNF-alpha in macrophages. PLOS One. 2013;8(12):e84107. doi: 10.1371/journal.pone.0084107, PMID 24358331.

18. Correa H, Valenzuela AL, Ospina LF, Duque C. Anti-inflammatory effects of the gorgonian Pseudopterogorgia elisabethae collected at the Islands of providencia and San Andres (SW Caribbean). J Inflamm (Lond). 2009;6:5. doi: 10.1186/1476-9255-6-5, PMID 19284567.

19. Abdelkarem FM, Desoky EK, Nafady AM, Allam AE, Mahdy A, Ashour A. Two new polyhydroxylated steroids from Egyptian soft coral Heteroxenia fuscescens (Fam Xeniidae). Nat Prod Res. 2021;35(2):236-43. doi: 10.1080/14786419.2019.1624958, PMID 31170807.

20. Yin FZ, Yang M, Li SW, Wu MJ, Huan XJ, Miu ZH. Two new hydroperoxy steroids from the South China Sea gorgonian Rumphella sp. Steroids. 2020 Mar;155:108558. doi: 10.1016/j.steroids.2019.108558, PMID 31866544.

21. Nam NH, Ngoc NT, Hanh TT, Cuong NX, Thanh NV, Thao DT. Cytotoxic steroids from the Vietnamese gorgonian Verrucella corona. Steroids. 2018;138:57-63. doi: 10.1016/j.steroids.2018.06.006, PMID 30018002.

22. He YQ, Lee Caplan SL, Scesa P, West LM. Cyclized 9,11-secosterol enol-ethers from the gorgonian Pseudopterogorgia americana. Steroids. 2017 Sep;125:47-53. doi: 10.1016/j.steroids.2017.06.008, PMID 28648587.

23. Chang YC, Hwang TL, Kuo LM, Sung PJ. Pinnisterols D–J, new 11-acetoxy-9,11-secosterols with a 1,4-quinone moiety from formosan gorgonian coral Pinnigorgia sp. (Gorgoniidae). Mar Drugs. 2017;15(1):11. doi: 10.3390/md15010011, PMID 28067822.

24. Cao F, Shao C, Wang M, Zhang X, Wang C. Steroids. Steroids. 2015;12:1068.

25. Deghrigue M, Festa C, Ghribi L, D Auria MV, De Marino S, Ben Jannet H. Anti-inflammatory and analgesic activities with gastroprotective effect of semi-purified fractions and isolation of pure compounds from Mediterranean gorgonian Eunicella singularis. Asian Pac J Trop Med. 2015;8(8):606-11. doi: 10.1016/j.apjtm.2015.07.019, PMID 26321512.

26. Chung HM, Hong PH, Su JH, Hwang TL, Lu MC, Fang LS. Bioactive compounds from a gorgonian coral Echinomuricea sp. (Plexauridae). Mar Drugs. 2012;10(5):1169-79. doi: 10.3390/md10051169, PMID 22822364.

27. Penez N, Culioli G, Perez T, Briand JF, Thomas OP, Blache Y. Antifouling properties of simple indole and purine alkaloids from the Mediterranean gorgonian Paramuricea clavata. J Nat Prod. 2011;74(10):2304-8. doi: 10.1021/np200537v, PMID 21939218.

28. Penez N, Culioli G, Perez T, Briand JF, Thomas OP, Blache Y. Antifouling properties of simple indole and purine alkaloids from the Mediterranean gorgonian Paramuricea clavata. J Nat Prod. 2011;74(10):2304-8. doi: 10.1021/np200537v, PMID 21939218.

29. Siva SD, Vijayakumar N, Jayaprakash R, Reddi NM. Review on isolation, identification and applications of Simarouba glauca plant. Rasayan J Chem. 2020;13(3):1580-8. doi: 10.31788/RJC.2020.1335793.

30. Lin YY, Lin SC, Feng CW, Chen PC, Su YD, Li CM. Anti-inflammatory and analgesic effects of the marine-derived compound excavatolide B isolated from the culture type formosan gorgonian Briareum excavatum. Mar Drugs. 2015;13(5):2559-79. doi: 10.3390/md13052559, PMID 25923315.

31. Chung HM, Hong PH, Su JH, Hwang TL, Lu MC, Fang LS. Bioactive compounds from a gorgonian coral Echinomuricea sp. (Plexauridae). Mar Drugs. 2012;10(5):1169-79. doi: 10.3390/md10051169, PMID 22822364.

32. Chung HM, Wang WH, Hwang TL, Chen JJ, Fang LS, Wen ZH. Rumphellols A and B, new caryophyllene sesquiterpenoids from a formosan gorgonian coral, Rumphella antipathies. Int J Mol Sci. 2014;15(9):15679-88. doi: 10.3390/ijms150915679, PMID 25192289.

33. Gonzalez Y, Doens D, Santamaria R, Ramos M, Restrepo CM, Barros De Arruda LB. A pseudopterane diterpene isolated from the octocoral Pseudopterogorgia acerosa inhibits the inflammatory response mediated by TLR-ligands and TNF-alpha in macrophages. PLOS One. 2013;8(12):e84107. doi: 10.1371/journal.pone.0084107, PMID 24358331.

34. Deghrigue M, Festa C, Ghribi L, D’Auria MV, De Marino S, Ben Jannet H. Anti-inflammatory and analgesic activities with gastroprotective effect of semi-purified fractions and isolation of pure compounds from Mediterranean gorgonian Eunicella singularis. Asian Pac J Trop Med. 2015;8(8):606-11. doi: 10.1016/j.apjtm.2015.07.019, PMID 26321512.

35. Abdelkarem FM, Desoky EK, Nafady AM, Allam AE, Mahdy A, Ashour A. Two new polyhydroxylated steroids from Egyptian soft coral Heteroxenia fuscescens (Fam.; Xeniidae). Nat Prod Res. 2021;35(2):236-43. doi: 10.1080/14786419.2019.1624958, PMID 31170807.

36. Thambidurai Y, Habeeb S, Kizhakudan J, DS. Screening of bioactive compounds from unknown marine sponges collected from kovalam, Chennai. Asian J Pharm Clin Res. 2017;10(5):231-6. doi: 10.22159/ajpcr.2017.v10i5.17347.

37. Sharifi S, Safaeian S. Anti-inflammatory effect of lipid extract of sea pen (Virgularia gustaviana) in mice. Asian J Pharm Clin Res. 2015;8(2):332-4.

38. Sharma S, Upadhyay RK. Coelenterate toxins its pharmaceutical and therapeutic effects. Int J Curr Pharm Sci. 2021;13(6):11-9. doi: 10.22159/ijcpr.2021v13i6.1912.

39. Bhadekar NS, Zodape GV. Original article bioactive compounds from sponge Suberites carnosus (Johnston) collected from West Coast of Mumbai, India. Int J Pharm Pharm Sci. 2021;13(6):41-51. doi: 10.22159/ijpps.2021v13i6.40794.