NEUROPROTECTIVE EFFECT OF 7,3´-DIHYDROXYFLAVONE AGAINST PACLITAXEL-INDUCED NEUROTOXICITY IN SH-SY5Y NEUROBLASTOMA CELL LINE: AN IN VITRO MODEL

KEERTHANA VIJAYARAJAN; KAVITHA RAMASAMY; KRANTHI KARUNAI KADAL

doi:10.22159/ajpcr.2025v18i3.53659

Authors

KEERTHANA VIJAYARAJAN Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Chennai, Tamil Nadu, India https://orcid.org/0009-0003-5841-6623
KAVITHA RAMASAMY Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Chennai, Tamil Nadu, India
KRANTHI KARUNAI KADAL Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Chennai, Tamil Nadu, India

DOI:

https://doi.org/10.22159/ajpcr.2025v18i3.53659

Keywords:

7,3´-Dihydroxyflavone, SH-SY5Y cells, Tumour necrosis factor-alpha, Interleukin-6

Abstract

Objectives: Paclitaxel (PT) induced peripheral neuropathy is associated with the neurotoxic effects of this chemotherapeutic drug. As a result, the need for natural substances that can mitigate neural toxicity is rising. This study investigated the Neuroprotective effects of 7,3´-dihydroxyflavone (7,3´-DHF) on PT-induced neurotoxicity in SH-SY5Y neuroblastoma cells.

Methods: The cells were exposed to a 1 μM concentration of PT, and the protective effects of various concentrations of 7,3´-DHF were evaluated. The reactive oxygen species (ROS) level in SH-SY5Y cells was measured using confocal fluorescence microscopy. The concentration of proinflammatory cytokines, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) was determined by enzyme-linked immunosorbent assay.

Results: Treatment of SH-SY5Y cells with 1 μM PT significantly reduced cell viability to 19±4.8%. However, treatment with 7,3´-DHF in PT-exposed cells elevated SH-SY5Y cell viability in a dose-dependent manner, with viability reaching 55.34±3.8% and 83.93±4.1% at 7,3´-DHF concentrations of 75 μg/mL and 100 μg/mL, respectively. PT exposure elevated ROS levels in SH-SY5Y cells, but the presence of 7,3´-DHF reduced ROS levels against PT-induced toxicity. In addition, treatment with 7,3´-DHF lowered the increased levels of IL-6 and TNF-α induced by PT exposure.

Conclusion: 7,3´-DHF effectively preserved the viability of SH-SY5Y cells under PT-induced toxicity. This protective effect was mediated through the suppression of ROS production and the attenuation of proinflammatory cytokine release, highlighting its potential as a neuroprotective agent.

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