IN VITRO ASSESSMENT OF ANTIOXIDANT AND ANTICANCER ACTIVITIES IN MURRAYA KOENIGII (L.): A PHYTOCHEMICAL APPROACH
DOI:
https://doi.org/10.22159/ajpcr.2026v19i4.57080Keywords:
Murraya koenigii (L.),, Leaves, In vitro anticancer, Antioxidant activities, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide assay,, Michigan Cancer Foundation-7 cell linesAbstract
Objectives: This study presents phytochemical screening and in vitro assessment of antioxidant and anticancer activity of Murraya koenigii (L.) leaf extract and isolated molecule MKLE-01.
Methods: The ethanol extract was screened for phytochemical analysis by phytochemical screening tests. The antioxidant activity of ethanol extract of M. koenigii and isolated compounds (MKLE-01) was evaluated by the 1-diphenyl-2-picrylhydrazyl method, and anticancer activity was carried out by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) assay method.
Results: The phytochemical screening of ethanol extract showed the presence of flavonoids, alkaloids, glycosides, phytosterols, and glycosides. In terms of their antioxidant activity, our findings demonstrated the ethanolic extracts’ significant in vitro free-radical scavenging capabilities. The extract and isolated compound half maximal inhibitory concentration (IC50) values of antioxidant activity were found at 154±0.202 μg/mL and 53±0.002 μg/mL, respectively. In comparison with cisplatin (IC50=6±0.001 μg/mL), ethanol extract revealed a moderate and dose-dependent growth diminution of breast cancer cells in the MTT assay at dosages over 100 μg/mL, with an IC50 of 72±0.023 μg/mL. The isolated compound displayed a significant effect with an IC50 of 34±0.002 μg/mL.
Conclusion: The results we obtained demonstrate that isolated compounds and ethanolic extract could represent promising anticancer agents and effective antioxidants.
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1. Das BM, Petruzzello SJ. The use of active living every day to improve mass transit district employees’ physical activity affect and enjoyment. Int J Health Promot Educ. 2015;53(3):147-55. doi: 10.1080/14635240.2014.978349
2. Tachibana Y, Kikuzaki H, Lajis NH, Nakatani N. Antioxidative activity of carbazoles from Murraya koenigii leaves. J Agric Food Chem. 2001;49(11):5589-94. doi: 10.1021/jf010621r, PMID 11714364
3. Naik SK, Mohanty S, Padhi A, Pati R, Sonawane A. Evaluation of antibacterial and cytotoxic activity of Artemisia nilagirica and Murraya koenigii leaf extracts against mycobacteria and macrophages. BMC Complement Altern Med. 2014;41(7):87.
4. Tachibana Y, Kikuzaki H, Lajis NH, Nakatani N. Comparison of antioxidative properties of carbazole alkaloids from Murraya koenigii leaves. J Agric Food Chem. 2003;51(22):6461-7. doi: 10.1021/ jf034700+, PMID 14558763
5. Ismail A, Noolu B, Gogulothu R, Perugu S, Rajanna A, Babu SK. Cytotoxicity and proteasome inhibition by alkaloid extract from Murraya koenigii leaves in breast cancer cells-molecular docking studies. J Med Food. 2016;19(12):1155-65. doi: 10.1089/jmf.2016.3767, PMID 27982755
6. Begum SA, Rani SJ, Banu A, Pavani A, Yeruva V. Statistics of cancer, 2020 in Indian states: A review on the report from national cancer registry programme. Asian J Pharm Clin Res. 2021;14(6):36-42. doi: 10.22159/ajpcr.2021v14i6.41616
7. Sukandar EY, Kurniati NF, Wikaningtyas P, Agprikani D. Antibacterial interaction of combination of ethanolic extract of Zingiber officinale var rubrum rhizome, Boesenbergia pandurata rhizome, and Stevia rebaudiana leaves with certain antibiotics against infectious mouth microbial. Asian J Pharm Clin Res. 2016;9(1):332-5.
8. Biswas AK, Kondaiah N, Anjaneyulu AS. Effect of spice mix and curry leaf (Murraya koenigii) powder on the quality of raw meat and precooked chicken patties during refrigeration storage. J Food Sci Technol. 2006;43:438-41.
9. Das AK, Rajkumar V, Dwivedi DK. Antioxidant effect of curry leaf (Murraya koenigii) powder on quality of ground and cooked goat meat. Int Food Res J. 2011;18:559-65.
10. Mohanta R, Roy S, Ghorai S, Banik S, Lohar A, Thapa S. Omics technologies in grapevine stress biology: Bridging molecular insights and sustainable viticulture under climate changes. Physiol Mol Plant Pathol. 2025 Sep 11;140:102942.
11. Patil S, Kadam K, Gupta A. Antifungal potential of a phytoelemental ointment: A combined study of Ocimum sanctum and alum. Int J Pharm Pharm Sci. 2025;7(2):28-35. doi: 10.33545/26647222.2025.v7.i2a.190
12. Singh B, Mohanty D, Upadhyay AK. Impact of genomics in improvement of rice bean production and productivity: An orphan crop. In: Genomics of Plant-Pathogen Interaction and the Stress Response. United States: CRC Press; 2023 Sep 25. p. 64-73.
13. Sumalatha D, Nithya TG. In vitro anti-oxidant and anticancer activity Of Murraya koenigii against human colon cancer Ht- 29 cell lines. Asian J Pharm Clin Res. 2014 Feb 1;7:83-6.
14. Madhura CM, Satyanarayana MS, Lavanya J, Kusuma GR. Isolation, identification and molecular characterization of carbapenemase producing Enterobacterales and Pseudomonas in a tertiary care hospital. Int J Curr Pharm Rev Res. 2025;17:526-32.
15. Sakhare DT. Synthesis, characterization and biological activity of divalent transition metal complexes of 3-{((4,6-dihydroxy pyrimidin-2- yl)Imino)methyl}Napthalen-2-ol. Int J Curr Res. 2025;17(9):34977-82.
16. Meena S, Rejeev Kumar S, Dwivedi V, Kumar Singh A, Chanotiya CS, Akhtar MQ. Transcriptomic insight into terpenoid and carbazole alkaloid biosynthesis, and functional characterization of two terpene synthases in curry tree (Murraya koenigii). Sci Rep. 2017;7:44126.
17. Singh S, Ahuja A, Murti Y, Khaliq A. Phyto-pharmacological review on Murraya koenigii (L.) Spreng: As an indigenous plant of India with high medicinal potential. Chem Biodivers. 2023 Jul;20(7):e202300483. doi: 10.1002/cbdv.202300483, PMID 37269458
18. Gahlawat DK, Jakhar S, Dahiya P. Murraya koenigii (L.) Spreng: An ethnobotanical, phytochemical and pharmacological review. J Pharmacogn Phytochem. 2014;3(3):109-19.
19. Balakrishnan R, Vijayraja D, Jo SH, Ganesan P, Su-Kim I, Choi DK. Medicinal profile, phytochemistry, and pharmacological activities of Murraya koenigii and its primary bioactive compounds. Antioxidants (Basel). 2020 Jan 24;9(2):101. doi: 10.3390/antiox9020101, PMID 31991665
20. Tan SP, Ali AM, Nafiah MA, Awang K, Ahmad K. Isolation and cytotoxic investigation of new carbazole alkaloids from Murraya koenigii (Linn.) Spreng. Tetrahedron. 2015;71(23):3946-53. doi: 10.1016/j.tet.2015.04.037
21. Iman V, Mohan S, Abdelwahab SI, Karimian H, Nordin N, Fadaeinasab M. Anticancer and anti-inflammatory activities of girinimbine isolated from Murraya koenigii. Drug Des Dev Ther. 2017;11:103-21. doi: 10.2147/dddt.s115135, PMID 28096658
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