PREPUPAE OIL FOR COSMETIC APPLICATION AND ANTI-HYALURONIDASE ACTIVITY-NANOEMULSIONS OF BLACK SOLDIER FLY (HERMETIA ILLUCENS)

ADHITYA JESSICA; SYIFA AINA; RESTI RAHAYU; RINI AGUSTIN

doi:10.22159/ijap.2025.v17s1.02

Authors

ADHITYA JESSICA Department of Pharmaceutics, Faculty of Pharmacy, Universitas Andalas, Padang-25163, Indonesia
SYIFA AINA Bachelor Program, Faculty of Pharmacy, Universitas Andalas, Padang-25163, Indonesia
RESTI RAHAYU Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang-25163, Indonesia
RINI AGUSTIN Department of Pharmaceutics, Faculty of Pharmacy, Universitas Andalas, Padang-25163, Indonesia https://orcid.org/0009-0001-8289-3011

DOI:

https://doi.org/10.22159/ijap.2025.v17s1.02

Keywords:

Nanoemulsion, Linoleic acid, Black soldier fly prepupae, Anti-hyaluronidase

Abstract

Objective: This study aimed to develop and evaluate Black Soldier Fly (BSF) (Hermetia illucens) prepupae oil nanoemulsions for cosmetic applications, focusing on their ability to inhibit the hyaluronidase enzyme.

Methods: The formulation process involved optimizing the combination of surfactant (Tween 80) and cosurfactant (PEG 400) using Simplex Lattice Design (SLD), with transmittance and pH as crucial parameters. A coarse oil-in-water (o/w) emulsion was prepared by mixing the oil and aqueous phases using a high-shear homogenizer. This emulsion was then subjected to ultrasonication with a probe sonicator to achieve the desired droplet size and stability. The physicochemical properties of the resulting nanoemulsions were characterized using appropriate analytical instruments, including physical appearance, pH value, particle size, polydispersity index (PDI), and zeta potential. Stability testing was conducted through heating-cooling cycling and centrifugation, monitoring changes in the formulations over time. Anti-hyaluronidase activity was assessed for anti-aging.

Results: The selected nanoemulsion, PP2, consisting of 53.5% Tween 80, 24.5% PEG 400, 6% distilled water, and 16% BSF prepupae oil, exhibited a droplet size of 307.8 nm, a polydispersity index of 0.656, and a zeta potential of-40 mV. The BSF prepupae oil has an IC₅₀ value of 0.173%, while the IC₅₀ values of BSF prepupae oil in nanoemulsion PP1 and PP2 are 0.053% and 0.066%.

Conclusion: PP2 demonstrated superior stability and anti-hyaluronidase activity (IC₅₀: 0.066%) compared to BSF prepupae Oil (IC₅₀: 0.173%), making it the most effective formulation.

References

Guilliet J, Baudouin G, Pollet N, Filee J. What complete mitochondrial genomes tell us about the evolutionary history of the black soldier fly, Hermetia illucens. BMC Ecol Evol. 2022 Dec 1;22(1):72. doi: 10.1186/s12862-022-02025-6, PMID 35650519.

Riolo K, Rotondo A, La Torre GL, Marino Y, Franco GA, Crupi R. Cytoprotective and antioxidant effects of hydrolysates from black soldier fly (Hermetia illucens). Antioxidants (Basel). 2023 Feb 18;12(2):519. doi: 10.3390/antiox12020519, PMID 36830077.

Phongpradist R, Semmarath W, Kiattisin K, Jiaranaikulwanitch J, Chaiyana W, Chaichit S. The in vitro effects of black soldier fly larvae (Hermitia illucens) oil as a high-functional active ingredient for inhibiting hyaluronidase, anti-oxidation benefits, whitening, and UVB protection. Front Pharmacol. 2023 Sep 20;14(Sep):1243961. doi: 10.3389/fphar.2023.1243961, PMID 37799972.

Caligiani A, Marseglia A, Leni G, Baldassarre S, Maistrello L, Dossena A. Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin. Food Res Int. 2018 Mar;105:812-20. doi: 10.1016/j.foodres.2017.12.012, PMID 29433277.

Lai‐Foenander AS, Kuppusamy G, Manogoran J, Xu T, Chen Y, Tang SY. Black soldier fly (Hermetia illucens L.): a potential small mighty giant in the field of cosmeceuticals [Hermetia illucens L]. Health Sci Rep. 2024 Jun 2;7(6):e2120. doi: 10.1002/hsr2.2120, PMID 38831777.

Shin SH, Lee YH, Rho NK, Park KY. Skin aging from mechanisms to interventions: focusing on dermal aging. Front Physiol. 2023;14:1195272. doi: 10.3389/fphys.2023.1195272, PMID 37234413.

Papakonstantinou E, Roth M, Karakiulakis G. Hyaluronic acid: a key molecule in skin aging. Derm Endocrinol. 2012 Jul 27;4(3):253-8. doi: 10.4161/derm.21923, PMID 23467280.

Rahayu R, Utari SD, Santoso P, Zaini E, Jessica A. Effectiveness of black soldier fly [Hermetia illucens] prepupa oil emulgel for burn wound recovery. Trop J Nat Prod Res. 2024;8(3):6589-93. doi: 10.26538/tjnpr/v8i3.17.

Santos Filipe M, Kastelic A, Ntungwe E, Almeida C, Diaz Lanza AM, Princiotto S. Lipidic extracts from Hermetia illucens larvae as potential ingredients for dermocosmetic applications; 2022.

Che Marzuki NH, Wahab RA, Abdul Hamid M. An overview of nanoemulsion: concepts of development and cosmeceutical applications. Biotechnol Biotechnol Equip. 2019 Jan 1;33(1):779-97. doi: 10.1080/13102818.2019.1620124.

Chou TH, Nugroho DS, Cheng YS, Chang JY. Development and characterization of nanoemulsions based on oil extracted from black soldier fly larvae. Appl Biochem Biotechnol. 2020 May 18;191(1):331-45. doi: 10.1007/s12010-019-03210-y, PMID 31853873.

Tayal S, Tiwari P, Pratap Singh U, Dubey S, Vishwakarma R. A review on formulation, characterization and applications of nanoemulsion. AJAST. 2024;8(1):67-84. doi: 10.38177/ajast.2024.8106.

Dewanti R, Ariyadi B, Martien R, Zuprizal Z. Self-nanoemulsifying drug delivery system of black soldier fly (Hermetia illucens) oil: optimization, formulation, and characterization. J Appl Pharm Sci. 2024;14(2):192-9. doi: 10.7324/JAPS.2024.153961.

Romes NB, Abdul Wahab R, Abdul Hamid M, Oyewusi HA, Huda N, Kobun R. Thermodynamic stability, in vitro permeability, and in silico molecular modeling of the optimal Elaeis guineensis leaves extract water-in-oil nanoemulsion. Sci Rep. 2021 Oct 21;11(1):20851. doi: 10.1038/s41598-021-00409-0, PMID 34675286.

Tu PT, Tawata S. Anti-oxidant, anti-aging, and anti-melanogenic properties of the essential oils from two varieties of alpinia zerumbet. Molecules. 2015 Sep 14;20(9):16723-40. doi: 10.3390/molecules200916723, PMID 26389869.

Heryani H. Penentuan kualitas degummed bleached palm oil (dbpo)dan refined bleached deodorized palm oil (RBDPO) dengan pemberian bleachingearth pada skala industri. J Tek Ind Pert. 2019;29(1):11-8. doi: 10.24961/j.tek.ind.pert.2019.29.1.11.

Helsawati H, Ratnapuri PH, Fitriana M. Formulation and evaluation of nanoemulsion Kelakai (Stenochlaena palustris) herbs with composition of Smix (Tween 80 and glycerin) and pine oil. Borneo J Pharm. 2023 Nov 30;6(4):406-16. doi: 10.33084/bjop.v6i4.3817.

Song R, Lin Y, Li Z. Ultrasonic-assisted preparation of eucalyptus oil nanoemulsion: process optimization, in vitro digestive stability, and anti-Escherichia coli activity. Ultrason Sonochem. 2022 Jan;82:105904. doi: 10.1016/j.ultsonch.2021.105904, PMID 34979457.

Bernardi DS, Pereira TA, Maciel NR, Bortoloto J, Viera GS, Oliveira GC. Formation and stability of oil-in-water nanoemulsions containing rice bran oil: in vitro and in vivo assessments. J Nanobiotechnology. 2011;9(1):44. doi: 10.1186/1477-3155-9-44, PMID 21952107.

Gonzalez E, Aponte Rivera C, Zia RN. Impact of polydispersity and confinement on diffusion in hydrodynamically interacting colloidal suspensions. J Fluid Mech. 2021 Oct 25;925:A35. doi: 10.1017/jfm.2021.563.

Suryani SMH, Sahumena MH, Mabilla SY, Ningsih SR, Adjeng AN, Aswan M. Preparation and evaluation of physical characteristics of vitamin E nanoemulsion using virgin coconut oil (VCO) and olive oil as oil phase with variation concentration of tween 80 surfactant. Res J Pharm Technol. 2020;13(7):3232. doi: 10.5958/0974-360X.2020.00572.7.

Hidayat M, Sarmadivaleh M, Derksen J, Vega Maza D, Iglauer S, Vinogradov J. Zeta potential of a natural clayey sandstone saturated with carbonated NaCl solutions at supercritical CO2 conditions. Geophys Res Lett. 2022 Aug 16;49(15). doi: 10.1029/2022GL099277.

Manaia EB, Abuçafy MP, Chiari Andreo BG, Silva BL, Oshiro Junior JA, Chiavacci LA. Physicochemical characterization of drug nanocarriers. Int J Nanomedicine. 2017;12:4991-5011. doi: 10.2147/IJN.S133832, PMID 28761340.

Mohammed NK, Muhialdin BJ, Meor Hussin AS. Characterization of nanoemulsion of Nigella sativa oil and its application in ice cream. Food Sci Nutr. 2020 Jun 21;8(6):2608-18. doi: 10.1002/fsn3.1500, PMID 32566178.

Yanasan N, Wangkananon W, Natakankitkul S, Kiattisin K. Nanoemulsions containing passiflora quadrangularis L. fruit extracts for cosmetic application and skin efficacy study. Cosmetics. 2024 Apr 4;11(2):57. doi: 10.3390/cosmetics11020057.