IMPROVED WOUND HEALING ACTIVITY THROUGH SYNERGISTIC APPROACH OF PUMPKIN SEED OIL AND CURCUMIN LOADED MODEL IN MICROGEL

MARGRET CHANDIRA RAJAPPA; AJITH KANNAN; NAGASUBRAMANIAN VENKATASUBRAMANIAM; LOKESH VENKATACHALAM; SELVARAGAVAN KARNAN; DOMINIC ANTONYSAMY

doi:10.22159/ijap.2025v17i5.54322

Authors

MARGRET CHANDIRA RAJAPPA Department of Pharmaceutics, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (DU), Ariyanoor, Salem-636308, India https://orcid.org/0000-0001-6364-2340
AJITH KANNAN Department of Pharmaceutics, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (DU), Ariyanoor, Salem-636308, India https://orcid.org/0009-0001-7435-5096
NAGASUBRAMANIAN VENKATASUBRAMANIAM Department of Pharmaceutics, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (DU), Ariyanoor, Salem-636308, India https://orcid.org/0009-0000-2957-1892
LOKESH VENKATACHALAM Department of Pharmaceutics, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (DU), Ariyanoor, Salem-636308, India https://orcid.org/0009-0003-2115-5300
SELVARAGAVAN KARNAN Department of Pharmaceutics, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (DU), Ariyanoor, Salem-636308, India https://orcid.org/0009-0009-0113-555X
DOMINIC ANTONYSAMY Department of Engineering, Sona College of Technology, Salem-636005, India https://orcid.org/0000-0002-0015-9690

DOI:

https://doi.org/10.22159/ijap.2025v17i5.54322

Keywords:

Curcumin, Wound healing, Lipophilic encapsulation, Microgel, Pumpkin seed oil

Abstract

Objective: This study aimed to develop a curcumin microgel with lipophilic encapsulation using pumpkin seed oil to enhance wound healing.

Methods: Curcumin was analyzed using ultraviolet-visible (UV-Vis) spectrophotometry, and microgels (CP1-CP6) were formulated with pumpkin seed oil encapsulation. Physicochemical tests were performed, and the optimized formulation was selected based on diffusion kinetics. The optimized formulation was subjected to wound healing studies through scratch wound heal assay.

Results: The calibration curve of curcumin showed a correlation coefficient>0.973. Drug-excipient studies revealed excellent compatibility. The optimized formulation, CP5 had the lowest zeta potential and highest viscosity, spreadability, and extrudability. CP5 released 94.59% of curcumin in 8 h, following the Korsmeyer-Peppas model. The minimum effective concentration promoting complete wound healing was 50µg/ml in L6 cell lines. CP5 remained stable for three months at 40±2 °C/75±5% RH.

Conclusion: Pumpkin seed oil effectively encapsulated curcumin, enhancing its wound healing potential. This microgel formulation showed promise for pharmaceutical applications, and future studies can investigate its pharmacodynamics and wound healing mechanisms.

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