ETHYL ACETATE FRACTION OF NIGELLA SATIVA HAS ANTIBACTERIAL ACTIVITY AND THE PROFILE OF ITS BIOAUTOGRAPHY
DOI:
https://doi.org/10.22159/ijap.2025.v17s2.01Keywords:
Antibacterial, Bioautography, Nigella sativa, Ethyl acetate fractionAbstract
Objective: Pathogenic microorganisms, including bacteria, viruses, fungi, and parasites, are sources of infectious diseases. It is known that Nigella sativa contains nigellimine, nigellimine-N-oxide, nigellidine, and nigellicine, as well as other active alkaloid compounds. The purpose of this study was to examine the antibacterial activity and bioautography of the ethyl acetate fraction of Nigella sativa against Bacillus cereus, Shigella sonnei, Streptococcus mutans, and Klebsiella pneumoniae.
Methods: Thin Layer Chromatography (TLC) was used in the bioautography analysis, and the disc diffusion technique was used to test the antibacterial activity. The concentrations of the ethyl acetate fractions were 20%, 40%, 60%, and 80%.
Results: The ethyl acetate fraction of Nigella sativa showed strong antibacterial activity against a number of microorganisms studied. According to the data, the inhibition zone was the best at a concentration of 80%. The diameters of the inhibition zones for the following bacteria were measured: 11.08±0.38 mm for Klebsiella pneumoniae, 12.50±0.50 mm for Shigella sonnei, and 17.67±0.58 mm for Bacillus cereus, covering a disc diameter of 6.00 mm. The findings of the bioautography test indicated that the alkaloid component is most likely the active ingredient causing the antibacterial activity.
Conclusion: Based on this research, the ethyl acetate fraction of Nigella sativa can be used as a natural antibacterial agent.
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