IN SILICO, IN VITRO AND IN VIVO STUDY OF BAWANG DAYAK (ELEUTHERINE BULBOSA (L) MERR.) POTENTIAL AS ANTI-CHOLESTEROL

NOVI ELISA; YUSTISIA DIAN ADVISTASARI; LIA KUSMITA; MUSTIKA ENDAH PRATIWI; CLAUDIUS HENDRAMAN BOLI TOBI

doi:10.22159/ijap.2025.v17s3.02

Authors

NOVI ELISA Department of Pharmacy, STIFAR Yayasan Pharmasi, Semarang, Central Java, Indonesia
YUSTISIA DIAN ADVISTASARI Department of Pharmacy, STIFAR Yayasan Pharmasi, Semarang, Central Java, Indonesia
LIA KUSMITA Department of Pharmacy, STIFAR Yayasan Pharmasi, Semarang, Central Java, Indonesia
MUSTIKA ENDAH PRATIWI Department of Pharmacy, Cenderawasih University, Jayapura, Papua, Indonesia https://orcid.org/0009-0003-7798-1732
CLAUDIUS HENDRAMAN BOLI TOBI Department of Pharmacy, Cenderawasih University, Jayapura, Papua, Indonesia https://orcid.org/0009-0002-0047-8752

DOI:

https://doi.org/10.22159/ijap.2025.v17s3.02

Keywords:

Anti-cholesterol, Bawang dayak extract, In silico, In vitro, In vivo

Abstract

Objective: This study aimed to determine the potential of Bawang Dayak extract as an anti-cholesterol.

Methods: In silico study was carried out using Gas Chromatography-Mass Spectroscopy (GC-MS) analysis and continued with screening using Lipinski’s Rule of Five. In vitro study was carried out using a cholesterol reagent that read in Multimode Reader. In vivo study was carried out by cholesterol level measurement against egg yolk-induced rats. The doses chosen by initial dose orientation and prior efficacy study of Bawang Dayak extract anti-cholesterol effect.

Results: The results of in silico study using the Lipinski's Rule of Five was active compound in Bawang Dayak was Undecanoic Acid. Undecanoic acid is ethyl ester compound with Log P<5. Molecular validation produced reference ligand compounds on the IHW9 receptor. Based on previously oriented concentrations and doses, the highest percentage of cholesterol decrease was at 50 ppm (40.04 %) in vitro, and 250 mg/70 kgBW (24.70 %) in vivo.

Conclusion: Bawang Dayak has an activity as anti-cholesterol that proven by in silico, in vitro and in vivo study. The absence of evaluation focused on mechanistic studies and anti-cholesterol pathways becomes limitation of this study.

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