FORMULATION AND CYTOTOXICITY EVALUATION NANOEMULSION OF JERUJU LEAVES (ACANTHUS ILLICIFOLIUS L) ETHANOL EXTRACT ON T47D BREAST CANCER CELLS

HENI SETYOWATI ESTI RAHAYU; PUSPITA SEPTIE DIANITA; KARTIKA WIJAYANTI; PERDANA PRIYA HARESMITA

doi:10.22159/ijap.2025.v17s3.06

Authors

HENI SETYOWATI ESTI RAHAYU Department of Maternity Nursing, Faculty of Health Science, Universitas Muhammadiyah Magelang, Indonesia
PUSPITA SEPTIE DIANITA Department of Pharmacy, Faculty of Health Science, Universitas Muhammadiyah Magelang, Indonesia
KARTIKA WIJAYANTI Department of Maternity Nursing, Faculty of Health Science, Universitas Muhammadiyah Magelang, Indonesia
PERDANA PRIYA HARESMITA Department of Pharmacy, Faculty of Health Science, Universitas Muhammadiyah Magelang, Indonesia

DOI:

https://doi.org/10.22159/ijap.2025.v17s3.06

Keywords:

Acanthus Illicifolius L, Breast Cancer, Nanoemulsion, T-47D cells, In vitro

Abstract

Objective: The first cause of death in the world is cancer. The type of cancer that is often found in women is breast cancer. Various treatments and therapies have been carried out, but the incidence of cancer is increasing. This is caused by lifestyle, nutrition and environmental conditions. Cancer drugs are very toxic, expensive and resistant, so it is necessary to try other therapies that are safe, do not damage other body cells, and do not cause side effects, namely herbal or plant-based therapies. One plant that has potential as a breast cancer therapy is jeruju (Acanthus Illicifolius L) leaves. The aim of this research was to determine the cytotoxic effect of jeruju leaf extract (Acanthus Illicifolius L) on T47D breast cancer cells in a nanoemulsion preparation.

Methods: Extraction of jeruju leaves was carried out using 96% ethanol solvent, then cytotoxicity testing was carried out using the MTT test on T47D cell proliferation, then the IC₅₀ value was calculated using a linear regression equation and continued with the preparation and testing of nanoemulsion preparations.

Results: Jeruju leaf extract (Acanthus Illicifolius L) has a strong cytotoxic activity value measured by an IC₅₀ value of 1.357 µg/ml and can be packaged in a nanoemulsion preparation which has a particle size of 16.1 nm. These findings indicate that jeruju leaf extract is effective in inhibiting T47D cells in vitro.

Conclusion: Jeruju leaf extract (Acanthus Illicifolius L) has a strong cytotoxic activity value measured by an IC50 value of 1.357 µg/ml and can be packaged in a nanoemulsion preparation which has a particle size of 16.1 nm.

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