CLINICAL PARAMETERS OF CHITOSAN ORAL WOUND DRESSING FILM IN THE GINGIVAL INCISIONS OF WISTAR RATS (RATTUS NORVEGICUS)

HESTY NURCAHYANTI; IRMA ERVINA; AINI HARIYANI NASUTION; HARRY AGUSNAR; DENNY SATRIA

doi:10.22159/ijap.2025.v17s5.04

Authors

HESTY NURCAHYANTI Department of Periodontics, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
IRMA ERVINA Department of Periodontics, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
AINI HARIYANI NASUTION Department of Periodontics, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
HARRY AGUSNAR Department of Biology, Faculty of Mathematics and Natural Science, Universitas Sumatera Utara, Medan, Indonesia
DENNY SATRIA Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.22159/ijap.2025.v17s5.04

Keywords:

Oral wound dressing film, Chitosan, Wound healing

Abstract

Objective: Oral Wound Dressing Film (OWDF) prevents bacterial infections and protects newly formed tissue. The periodontal surgical treatment causes incised wounds, which may result in excessive pain. Periodontal dressings in film preparation are considered easier to apply. Chitosan is effective in accelerating wound healing because it’s mucoadhesive, biocompatible, biodegradable, antimicrobial, and non-toxic properties. The research aims to analyze the effectiveness of Oral Wound Dressing Chitosan Film on clinical Landry's Healing Index and vascularization in the wound healing process after wistar rat gingival incision.

Methods: In vivo experimental research with a post-test control group design of 27 male wistar rats in three treatment groups with oral wound dressing film chitosan 2.5%, Ora-Aid^®, and placebo. Ora-Aid^®used as positive control because it’s widely commercialize tomaintain the humidity in the oral environment and improve wound healing. Clinical parameters were examined with Landry's Healing Index and histological parameters of vascularization on d 3, 7, and 14. Data were analyzed using kruskal-wallis and mann-whitney.

Results: There were significant differences in vascularization between the groups on 3^rdd (4.20±0.200), 7^thd (5.60±0.600) and 14^thd (7.20±0.872) with p value<0.05. There were differences in vascularization (p<0.05) between the two groups except for vascularization 3^rdd, 7^thd and 14^thd between Oral Wound Dressing Film chitosan and Ora-Aid^® groups (p>0.05).

Conclusion: Clinical and vascularization parameters increased after the application of an Oral Wound Dressing Film containing chitosan in the post-gingival incision wound healing process in Wistar rats (Rattus norvegicus).

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