COMBINATION OF 10% PROPOLIS AND 0.8% HYALURONIC ACID GEL ACCELERATES OSTEOBLAST PROLIFERATION IN VITRO
DOI:
https://doi.org/10.22159/ijap.2025.v17s5.05Keywords:
Osteoblast proliferation, Propolis, Hyaluronic acid, Bone regenerationAbstract
Objective: In treating periodontitis, bone regeneration can be promoted by signaling molecules and antibacterial properties. Hyaluronic acid induces signaling molecules; however, it has limited antibacterial properties. Propolis has the advantage of activating signaling molecules and possesses potent antibacterial properties. This investigation aimed to evaluate the impact of a combination of 10% propolis and 0.8% hyaluronic acid on the proliferation of MC3T3-E1 osteoblasts.
Methods: In vitro experiments were conducted in three groups, each with five technical replicates: group A received 10% propolis and 0.8% hyaluronic acid gel (n = 5), group B received 0.8% hyaluronic acid gel (n = 5), and group C received 2% Sodium Carboxy-Methyl Cellulose (CMC-Na) gel (n = 5). The osteoblast count was assessed on days 1, 3, and 7 using the Cell Counting Kit (CCK)-8 assay. Data were analyzed using Two-Way ANOVA and Tukey’s High Significant Difference (HSD) test (p<0.05).
Results: The results indicated that the number of osteoblasts in group A was significantly higher than in groups B and C on days 3 and 7 (p<0.05). On day 3, the number of osteoblasts in group A was equivalent to that of group B on day 7 (p>0.05).
Conclusion: The combination of 10% propolis and 0.8% hyaluronic acid gel enhanced and expedited osteoblast proliferation in alveolar bone. The limitations of this study include the lack of in vivo validation and a relatively short observation period; hence, further studies are required prior to clinical translation.
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