EFFECTIVENESS OF CHITOSAN FILM ON NEUTROPHILS AND MACROPHAGES COUNTS IN THE HEALING OF GINGIVAL INSICION IN WISTAR RATS (RATTUS NORVEGICUS)
DOI:
https://doi.org/10.22159/ijap.2025.v17s5.10Keywords:
Chitosan film, Chitosan, Neutrophils, Macrophage, Wound healingAbstract
Objective: Incisional wounds caused by surgical interventions often result in complications, including infections. Chitosan is a promising material to promote healing. This study aimed is to determine the effectiveness of chitosan film as a periodontal dressing on the number of neutrophils and macrophages in chitosan film.
Methods: This in vivo experimental study used 36 rats with 3 mm gingival incisions. Rats were divided into three groups: chitosan film (treatment), Ora-Aid (positive control), and placebo (negative control). Dressings were applied twice daily for seven days. Rats were euthanized on days 1, 3, and 7 for neutrophil analysis, and on days 3, 7, and 14 for macrophage evaluation.
Results: The study showed that on day one, neutrophil counts were highest in the chitosan group, followed by the positive control, and lowest in the placebo group, with a significant difference (p<0.05). Neutrophil numbers decreased in all groups over time. By days 3 and 7, there was no significant difference between the Ora-Aid and chitosan groups (p>0.05). On day 3, macrophage counts were highest in the chitosan film group, followed by Ora-Aid and placebo, with a significant difference (p<0.05). By day 7, no significant difference was found between the chitosan and positive control groups (p>0.05).
Conclusion: Chitosan film demonstrates a higher number of neutrophils and macrophages during the early stages of healing compared to placebo. Therefore, chitosan film may be considered a potential wound dressing material to accelerate the healing process.
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