THE ROLE OF CITRUS RETICULATE ETHANOLIC LEAF EXTRACT IN RESTORING MEMORY IN MICE WITH HIGH-FAT DIET-INDUCED DEMENTIA

POOJA SHARMA; AJEET PAL SINGH; AMAR PAL SINGH

doi:10.22159/ijcpr.2025v17i4.7015

Authors

POOJA SHARMA Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R. E. C.), Jalandhar –Amritsar by pass, NH-1, Jalandhar-144011, Punjab, India
AJEET PAL SINGH Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R. E. C.), Jalandhar –Amritsar by pass, NH-1, Jalandhar-144011, Punjab, India
AMAR PAL SINGH Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R. E. C.), Jalandhar –Amritsar by pass, NH-1, Jalandhar-144011, Punjab, India

DOI:

https://doi.org/10.22159/ijcpr.2025v17i4.7015

Keywords:

Dementia, Citrus reticulata, Morris water maze (MWZ) test, Elevated plus maze (EPZ) test, T maze delayed alteration task, High fat diet, Piracetam

Abstract

Objective: To evaluate Citrus reticulate ethanolic leaf extract in restoring memory in mice with high-fat diet-induced dementia.

Methods: The experimental animals were divided into five groups, including the Naive Animal group, High Fat Diet (HFD) group, Piracetam+HFD group, HFD+Citrus reticulata, a low dose of 250 mg/kg was given orally, while a high dose of 500 mg/kg was administered via the same route. The factors used involve the Morris water maze (MWZ), elevated plus maze (EPZ), T maze delayed alteration task, and measurement of serum total cholesterol levels.

Results: This study examined the effects of Citrus reticulata ethanolic leaf extract on dementia. Two doses, a high dose (500 mg/kg; orally) and a low dose (250 mg/kg; orally), were chosen based on prior research, aiming to induce behavioral changes, particularly in learning and memory. The findings from the morris water maze, elevated plus maze, and t-maze tests suggest that the high dose (500 mg/kg; orally) of Citrus reticulata ethanolic leaf extract produced results comparable to the standard drug piracetam. However, the low dose (250 mg/kg; orally) did not exhibit any significant positive effects.

Conclusion: The study found that HFD impairs learning and memory, while Citrus reticulata extract treatment significantly improved cognitive functions in HFD-treated mice. The entire study suggests that Citrus reticulata leaves may have a memory-restorative role in HFD-induced dementia, and that Pregnane X receptors may be implicated.

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