PHARMACOLOGICAL EVALUATION OF ANTI-PARKINSON ACTIVITY OF DELPHINIUM DENUDATUM (JADWAR) FLOWERS ETHANOLIC EXTRACT IN MICE

AKASHDEEP; AJEET PAL SINGH; AMAR PAL SINGH

doi:10.22159/ijcpr.2025v17i6.7055

Authors

AKASHDEEP Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R. E. C.), Jalandhar –Amritsar bypass, NH-1, Jalandhar-144011, Punjab, India
AJEET PAL SINGH Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R. E. C.), Jalandhar –Amritsar bypass, NH-1, Jalandhar-144011, Punjab, India
AMAR PAL SINGH Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R. E. C.), Jalandhar –Amritsar bypass, NH-1, Jalandhar-144011, Punjab, India

DOI:

https://doi.org/10.22159/ijcpr.2025v17i6.7055

Keywords:

Parkinson's disease (PD), 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), Delphiniumdenudatum (Jadwar) flowers ethanolic extract, Rota rod test, Hanging test, Swimming test, Open field test, Actophotometer test, Elevated plus maze test, Catalepsy bar test, Neuroprotective activity

Abstract

Objective: This study aimed to evaluate the neuroprotective effects of ethanolic extracts of Delphinium denudatum (Jadwar) flowers in an MPTP-induced mouse model of Parkinson’s disease (PD).

Methods: Parkinsonism was induced in Swiss albino mice using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Mice were divided into five groups: control, negative control (MPTP), standard drug (Levodopa+Carbidopa), and two test groups receiving low (200 mg/kg) and high (400 mg/kg) doses of the ethanolic extract. The extract was administered orally for 7 d, followed by MPTP injections for another 7 d. Behavioral assessments were conducted using rota rod test, hanging test, forced swim test, open field test, actophotometer, elevated plus maze, and catalepsy bar test.

Results: The high-dose group (400 mg/kg) demonstrated significant improvement in motor coordination, grip strength, reduced immobility time, enhanced locomotor activity, and decreased cataleptic behavior compared to the MPTP group. These results were comparable to those observed in the standard treatment group, indicating a potential neuroprotective effect of the plant extract.

Conclusion: Delphinium denudatum ethanolic flower extract showed promising anti-parkinsonian activity by mitigating MPTP-induced motor and behavioral deficits in mice. This suggests its potential as a complementary therapeutic strategy for managing Parkinson’s disease, warranting further clinical exploration.

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