PREPARATION, EVALUATION AND IN VIVO STUDIES OF NOVEL HERBAL LIPOGEL OF OCIMUM TENUIFLORAM (L.) EXTRACT FOR ACNE TREATMENT

VANDANA SINGH; RAVINDER VERMA; VINEET MITTAL; DEEPAK KAUSHIK

doi:10.22159/ijap.2025v17i4.53761

Authors

VANDANA SINGH Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak-124001, Haryana, India https://orcid.org/0000-0001-9337-9328
RAVINDER VERMA Department of Pharmaceutical Sciences, Chaudhary Bansi Lal University, Bhiwani-127021, India https://orcid.org/0000-0003-3397-703X
VINEET MITTAL Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak-124001, Haryana, India
DEEPAK KAUSHIK Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak-124001, Haryana, India

DOI:

https://doi.org/10.22159/ijap.2025v17i4.53761

Keywords:

Ocimum tenuifloram (L.) herb, Liposomes, Antibacterial activity, In vivo studies, Stability studies

Abstract

Objective: The present research work was aimed to develop and design novel anti-acne herbal lipogel using extract of Ocimum tenuifloram (L.) leaves with ethanol injection method. It is the first time herbal lipogel containing liposomes that, improves the drug retention time and increases its therapeutic efficacy.

Methods: This research work included the preparation of liposome vesicles by Ethanol injection technique by using cholesterol, soya lecithin, ethanol and Ocimum tenuifloram Lamiaceae (L.) extract. The liposomal vesicles were optimized by Box Behken Design (BBD). The formulation of herbal lipogel of Ocimum tenuifloram (L.) extract shows maximum anti-bacterial results against Propionibacterium acne (p. acne) bacteria with 19.31 mm and 12.675 mm zone of inhibition when compared with marketed anti-acne formulation. The optimized size of liposomes with 198 nm was observed.

Results: By analysis of Gas chromatography-mass spectrometry (GC-MS) and High-performance liquid chromatography (HPLC) method, the presence of eugenol (32%) was determined. The optimized batch of liposome (F6) was selected with entrapment efficiency (EE%) 79.8% and zeta potential (ZP)-19.8mV.

Conclusion: Finally, it can be concluded that the prepared novel herbal lipogel of Ocimum tenuifloram (L.) extract was suitable in acne on the basis of in vivo studies. The optimized herbal lipogel formulation was safe and effective when compared with other marketed herbal formulations of acne.

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