SIMULTANEOUS METHOD DEVELOPMENT AND VALIDATION OF GABAPENTIN AND CARBAMAZEPINE IN RAT PLASMA USING LC-MS/MS AND ITS APPLICATION TO PHARMACOKINETIC STUDIES

Authors

  • P. SRIVIDYA Department of chemistry, University College of Sciences, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur (Dt), A. P., India
  • P. BHARATH Department of chemistry, University College of Sciences, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur (Dt), A. P., India
  • SK MUBEENA Department of chemistry, University College of Sciences, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur (Dt), A. P., India
  • B. SREE RAMUDU Department of Chemistry, Adikavi Nannaya University Campus, Tadepalligudem-534101, A. P., India
  • D. RAMACHANDRAN Department of chemistry, University College of Sciences, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur (Dt), A. P., India

DOI:

https://doi.org/10.22159/ijap.2026v18i2.56696

Keywords:

Gabapentin, Carbamazepine, LC-MS/MS, USFDA guidelines, Rat plasma

Abstract

Objective: For the bioanalytical method of gabapentin and carbamazepine using tiagabine as the internal standard (IS), a simple, rapid, accurate, specific, and repeatable Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) approach was devised.

Methods: A Symmetry C18 column (150x4.6 mm, 3.5µ) and mobile phase of 0.1% formic acid in water and acetonitrile (70:30, v/v) are used in this article to summarise the latest advancements in bioanalytical LC-MS/MS procedures.

Results: Analysis was carried out within 7 min over a good linear concentration range from 6.0ng/ml to 240ng/ml (r2= 0.9999±0.004) for Gabapentin and 2ng/ml to 80ng/ml (r2=0.9998± 0.003) for Carbamazepine. Accuracy, precision, recovery, matrix effect and stability results were found to be within the suitable limits.

Conclusion: When used successfully for the examination of rat pharmacokinetic studies, the application indicates that all system appropriateness, specificity, linearity, and accuracy metrics are in excellent compliance with USFDA requirements.

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Published

07-03-2026

How to Cite

SRIVIDYA, P., BHARATH, P., MUBEENA, S., RAMUDU, B. S., & RAMACHANDRAN, D. (2026). SIMULTANEOUS METHOD DEVELOPMENT AND VALIDATION OF GABAPENTIN AND CARBAMAZEPINE IN RAT PLASMA USING LC-MS/MS AND ITS APPLICATION TO PHARMACOKINETIC STUDIES. International Journal of Applied Pharmaceutics, 18(2), 455–463. https://doi.org/10.22159/ijap.2026v18i2.56696

Issue

Vol 18, Issue 2 (Mar-Apr), 2026

Section

Original Article(s)

Copyright (c) 2026 P. SRIVIDYA, P. BHARATH, SK. MUBEENA, B. SREE RAMUDU, D. RAMACHANDRAN

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