IN VITRO EVALUATION OF TETRACYCLINE-LOADED CHITOSAN CHIPS AGAINST PERIODONTAL PATHOGENS
DOI:
https://doi.org/10.22159/ijap.2025.v17s5.07Keywords:
Periodontal pathogens, Chip, Tetracycline, ChitosanAbstract
Objective: A periodontal pathogen is the main cause of periodontitis. Tetracycline has demonstrated its ability to impede the proliferation of periodontal pathogens. A chip is a device that employs a matrix to distribute drugs evenly into a polymer. The release of the drugs occurs through the process of drug diffusion. It possesses numerous benefits in terms of appealing physical characteristics for use within a pocket. Chitosan is a naturally occurring biopolymer with favorable characteristics for use as a drug delivery system.
Methods: Using solvent casting, chitosan-based periodontal chips containing 0.7% tetracycline were created. Scanning Electron Microscopy (SEM) and Fourier transform infrared (FTIR) were used to characterize the structure and morphology of the chitosan-based periodontal chip. The antibacterial performance was analyzed against A. actinomycetemcomitans, P. gingivalis, and F. nucleatum bacteria grown in an MHA medium.
Results: Based on the results of SEM analysis at 1000x magnification, tetracycline can be seen on the surface of chitosan, while FTIR analysis shows compatibility between chitosan and tetracycline. The average diameter of the inhibition zone of chitosan-based periodontal chips containing 0.7% tetracycline towards P. gingivalis, A. actinomycetemcomitans and F. nucleatum were 14.75±0.75 mm, 25.67±2.082 mm, and 35.33±0.764 mm. In tests on Aa and Fn bacteria, the inhibition zone was larger compared to the PerioChipâ. The ANOVA test revealed statistically significant variations in the average diameter of the inhibitory zone across different groups when tested against periodontal infections.
Conclusion: This research has shown potent antimicrobial activity of chitosan-based periodontal chips containing 0.7 % tetracycline against A. actinomycetemcomitans, P. gingivalis, and F. nucleatum. This reseaerch showed more microbial activity than PerioChipâ for A. actinomycetemcomitans and F. nucleatum.
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