ANTIDEPRESSANT ACTIVITY OF PERICARPIUM GRANATI PEELS IN ALBINO MICE-A BENEFICIAL HERBAL TREATMENT STRATEGY

AMAN SHARMA; AJEET PAL SINGH; AMAR PAL SINGH; MAMTA KUMARI

doi:10.22159/ijcpr.2025v17i6.7064

Authors

AMAN SHARMA Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R. E. C.), Jalandhar–Amritsar by pass, NH-1, Jalandhar-144011, Punjab, India
AJEET PAL SINGH Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R. E. C.), Jalandhar–Amritsar by pass, NH-1, Jalandhar-144011, Punjab, India
AMAR PAL SINGH Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R. E. C.), Jalandhar–Amritsar by pass, NH-1, Jalandhar-144011, Punjab, India
MAMTA KUMARI Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Ooty, The Nilgiris, Tamil Nadu, India

DOI:

https://doi.org/10.22159/ijcpr.2025v17i6.7064

Keywords:

Antidepressant activity, Pericarpium granati, Neurological disorder, Blood glucose, Glucosetolerance

Abstract

Objective: To evaluate Antidepressant Activity of ethanolic and aqueous peel extracts of Pericarpium Granatiin albino mice by using Forcedswim test (FST), Open field test (OFT) and tail suspension test (TST)) parameters.

Methods: For the study, 25–40 g adult Laca albino mice of either sex, age between 4-5 mo procuredfrom Lala Lajpat Rai University of veterinary and animal sciences, Hisar (125004),India and were housed in polypropylene cage. They were maintained under standard laboratoryconditions (temperature 25±2º C with 12/12h night/dark cycle), were fed with standard pelletdiet and water ad libitum.

Results: To evaluate the pharmacological effectiveness of various successive extracts of Pericarpium granati in Laca albino mice was investigatedin fourdifferent groups; Naive Control, Standard (Imipramine 10 mg/kg), Test Group-І, 200 mg/kg p. o. Pericarpium granati ethanolic extracts, Test Group-ІІ, 200 mg/kg p. o. Pericarpium granatiaqueous extracts. The numbers of parameters in relation to the depression were observed and thesignificant (P<0.01) decreased in the duration of immobility time were observed in Test Group-І(200 mg/kg) as compared to control group.

Conclusion: This study clearly demonstrated that ethanolic extract of Pericarpium granati might serve as apowerful antidepressant agent in adult Laca albino mice without causing any serious toxiceffects.

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References

1. Das MC, Srinivasa Rao ASR, Karuna Sri G. Antidepressant activity of brahmi in albino mice. J Clin Diagn Res. 2014;8(3):35-7. doi: 10.7860/JCDR/2014/7482.4098, PMID 24783074.

2. Nestler EJ, Barrot M, DiLeone RJ, Eisch AJ, Gold SJ, Monteggia LM. Neurobiology of depression. Neuron. 2002;34(1):13-25. doi: 10.1016/s0896-6273(02)00653-0, PMID 11931738.

3. Castagne V, Moser P, Porsolt RD. Behavioral assessment of antidepressant activity in rodents. Buccafusco JJ (ed). Methods of Behavior Analysis in Neuroscience. 2nd ed. Boca Raton (FL): CRC Press/Taylor & Francis; 2009.

4. Berton O, Nestler EJ. New approaches to antidepressant drug discovery: beyond monoamines. Nat Rev Neurosci. 2006;7(2):137-51. doi: 10.1038/nrn1846, PMID 16429123.

5. Brigitta B. Pathophysiology of depression and mechanisms of treatment. Dialogues Clin Neurosci. 2002;4(1):7-20. doi: 10.31887/DCNS.2002.4.1/bbondy, PMID 22033824.

6. Biological Sciences Curriculum Study, National Institutes of Health. Information about mental illness and the brain. In: NIH curriculum Supplement Series. Bethesda National Institutes of Health; 2007.

7. Sansone RA, Sansone LA. Dysthymic disorder: forlorn and overlooked? Psychiatry (Edgmont). 2009;6(5):46-51. PMID 19724735.

8. Hilty DM, Leamon MH, Lim RF, Kelly RH, Hales RE. A review of bipolar disorder in adults. Psychiatry (Edgmont). 2006 Sep;3(9):43-55. PMID 20975827.

9. Liu L, Liu C, Wang Y, Wang P, Li Y, Li B. Herbal medicine for anxiety depression and insomnia. Curr Neuropharmacol. 2015;13(4):481-93. doi: 10.2174/1570159x1304150831122734, PMID 26412068.

10. Yeung KS, Hernandez M, Mao JJ, Haviland I, Gubili J. Herbal medicine for depression and anxiety: a systematic review with assessment of potential psycho-oncologic relevance. Phytother Res. 2018;32(5):865-91. doi: 10.1002/ptr.6033, PMID 29464801.

11. Park SY, Na CS, Jeong WC, Lee JC. A literature study of Pericarpium Granati and cortex Betulae Platyphyllae. The Journal of Korean Oriental Medical Ophthalmology and Otolaryngology and Dermatology. 2012;25(3):13-33. doi: 10.6114/jkood.2012.25.3.013.

12. Bhowmik D, Gopinath H, Kumar BP, Kumar K. Medicinal uses of Punica granatum and its health benefits. J Pharmacogn Phytochem. 2013;1(5):28-35.

13. Kaur G, Jabbar Z, Athar M, Alam MS. Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food Chem Toxicol. 2006;44(7):984-93. doi: 10.1016/j.fct.2005.12.001, PMID 16426722.

14. Riaz A, Khan RA. Behavioral effects of Citrus limon and Punica granatum combinations in rats. Metab Brain Dis. 2017;32(1):123-31. doi: 10.1007/s11011-016-9884-0, PMID 27510713.

15. Shaygannia E, Bahmani M, Zamanzad B, Rafieian Kopaei M. A review study on Punica granatum L. J Evid Based Complementary Altern Med. 2016;21(3):221-7. doi: 10.1177/2156587215598039, PMID 26232244.

16. Cam M, Icyer NC, Erdogan F. Pomegranate peel phenolics: microencapsulation storage stability and potential ingredient for functional food development. LWT Food Sci Technol. 2014;55(1):117-23. doi: 10.1016/j.lwt.2013.09.011.

17. Grabez M, Skrbic R, Stojiljkovic MP, Rudic Grujic V, Paunovic M, Arsic A. Beneficial effects of pomegranate peel extract on plasma lipid profile fatty acids levels and blood pressure in patients with diabetes mellitus type-2: a randomized double-blind placebo controlled study. J Funct Foods. 2020;64:103692. doi: 10.1016/j.jff.2019.103692.

18. Wang BS, Leu KL, Huang GJ, Yeh CF, Tai HM, Ho WY. Protective effects of an aqueous Pericarpium Granati extract against inflammatory damage in mice. J Funct Foods. 2014;9:183-91. doi: 10.1016/j.jff.2014.04.022.

19. Sahebkar A, Ferri C, Giorgini P, Bo S, Nachtigal P, Grassi D. Effects of pomegranate juice on blood pressure: a systematic review and meta-analysis of randomized controlled trials. Pharmacol Res. 2017;115:149-61. doi: 10.1016/j.phrs.2016.11.018, PMID 27888156.

20. Suman M, Bhatnagar P. A review on proactive pomegranate one of the healthiest foods. Int J Chem Stud. 2019;7(3):189-94.

21. Ventura J, Alarcon Aguilar F, Roman Ramos R, Campos Sepulveda E, Reyes Vega ML, Daniel Boone Villa VD. Quality and antioxidant properties of a reduced sugar pomegranate juice jelly with an aqueous extract of pomegranate peels. Food Chem. 2013;136(1):109-15. doi: 10.1016/j.foodchem.2012.07.039, PMID 23017400.

22. Pahwa P, Goel RK. Absence of anticonvulsant activity in Asparagus adscendens Roxb. hydroethanolic root extract against acute pentylenetetrazol and maximal electroshock induced convulsion mice models. J Pharm Neg Results. 2016;7(1):25-8.

23. Toxicity–up AO. OECD guideline for testing of chemicals. Vol. 17. Paris, France: Organization for economic co-operation and development; 2001. p. 1.

24. Detke MJ, Johnson J, Lucki I. Acute and chronic antidepressant drug treatment in the rat forced swimming test model of depression. Exp Clin Psychopharmacol. 1997;5(2):107-12. doi: 10.1037//1064-1297.5.2.107, PMID 9234045.

25. Castagne V, Moser P, Roux S, Porsolt RD. Rodent models of depression: forced swim and tail suspension behavioral despair tests in rats and mice. Curr Protoc Pharmacol. 2010;49(1):5.8. doi: 10.1002/0471141755.ph0508s49, PMID 22294373.

26. Strekalova T, Spanagel R, Dolgov O, Bartsch D. Stress induced hyperlocomotion as a confounding factor in anxiety and depression models in mice. Behav Pharmacol. 2005;16(3):171-80. doi: 10.1097/00008877-200505000-00006, PMID 15864072.

27. Gutierrez Garcia AG, Contreras CM, Mendoza Lopez MR, Cruz Sanchez S, Garcia Barradas O, Rodriguez Landa JF. A single session of emotional stress produces anxiety in wistar rats. Behav Brain Res. 2006;167(1):30-5. doi: 10.1016/j.bbr.2005.08.011, PMID 16216347.

28. Ishola IO, Ochieng CO, Olayemi SO, Jimoh MO, Lawal SM. Potential of novel phytoecdysteroids isolated from Vitex doniana in the treatment depression: involvement of monoaminergic systems. Pharmacol Biochem Behav. 2014;127:90-100. doi: 10.1016/j.pbb.2014.11.005, PMID 25449355.

29. Patel SS, Mehta V, Changotra H, Udayabanu M. Depression mediates impaired glucose tolerance and cognitive dysfunction: a neuromodulatory role of rosiglitazone. Horm Behav. 2016;78:200-10. doi: 10.1016/j.yhbeh.2015.11.010, PMID 26631485.

30. Jahromy MH, Khakpour S, Khorgami Z. The antidepressant like effects of Punica granatum (Pomegranate) extract in mice. Chin Med. 2014;5:1-6. doi: 10.4236/cm.2014.51001.

31. Pauleti NN, Mello J, Siebert DA, Micke GA, De Albuquerque CA, Alberton MD. Characterisation of phenolic compounds of the ethyl acetate fraction from Tabernaemontana catharinensis and its potential antidepressant-like effect. Nat Prod Res. 2018;32(16):1987-90. doi: 10.1080/14786419.2017.1359167, PMID 28764559.

32. Ferreres F, Grosso C, Gil Izquierdo A, Valentao P, Andrade PB. Phenolic compounds from Jacaranda caroba (Vell.) A. DC: approaches to neurodegenerative disorders. Food Chem Toxicol. 2013;57:91-8. doi: 10.1016/j.fct.2013.03.012, PMID 23524314.

33. Valdes Sustaita B, Lopez Rubalcava C, Gonzalez Trujano ME, Garcia Viguera C, Estrada Camarena E. Aqueous extract of pomegranate alone or in combination with citalopram produces antidepressant-like effects in an animal model of menopause: participation of estrogen receptors. Int J Mol Sci. 2017;18(12):2643. doi: 10.3390/ijms18122643, PMID 29257042.

34. Naveen S, Siddalingaswamy M, Singsit D, Khanum F. Anti-depressive effect of polyphenols and omega-3 fatty acid from pomegranate peel and flax seed in mice exposed to chronic mild stress. Psychiatry Clin Neurosci. 2013;67(7):501-8. doi: 10.1111/pcn.12100, PMID 24152226.

35. Yankelevitch Yahav R, Franko M, Huly A, Doron R. The forced swim test as a model of depressive like behavior. J Vis Exp. 2015;(97):52587. doi: 10.3791/52587, PMID 25867960.

36. Pesarico AP, Birmann PT, Pinto R, Padilha NB, Lenardao EJ, Savegnago L. Short and long term repeated forced swim stress induce depressive like phenotype in mice: effectiveness of 3-[(4-chlorophenyl)selanyl]-1-methyl-1H-indole. Front Behav Neurosci. 2020;14:140. doi: 10.3389/fnbeh.2020.00140, PMID 33192355.

37. Shashikumara S, CP, Sibgatullah M. Evaluation of antidepressant activity of ethanolic extract of Alangium Salvifolium leaves in swiss albino mice. Biomed Pharmacol J. 2017;10(1):427-33. doi: 10.13005/bpj/1125.

38. Fuchs E, Fliugge G. Experimental animal models for the simulation of depression and anxiety. Dialogues Clin Neurosci. 2006;8(3):323-33. doi: 10.31887/DCNS.2006.8.3/efuchs, PMID 17117614.

39. Jansen AS, Van Nguyen XV, Karpitskiy V, Mettenleiter TC, Loewy AD. Central command neurons of the sympathetic nervous system: basis of the fight-or-flight response. Science. 1995;270(5236):644-6. doi: 10.1126/science.270.5236.644, PMID 7570024.

40. Lim SM, Park SH, Sharma N, Kim SS, Lee JR, Jung JS. Blood glucose regulation mechanism in depressive disorder animal model during hyperglycemic states. Brain Res Bull. 2016;124:116-22. doi: 10.1016/j.brainresbull.2016.03.014, PMID 27034116.

41. Cox DJ, Gonder Frederick L. Major developments in behavioral diabetes research. J Consult Clin Psychol. 1992;60(4):628-38. doi: 10.1037//0022-006x.60.4.628, PMID 1506511.

42. Berton O, Nestler EJ. New approaches to antidepressant drug discovery: beyond monoamines. Nat Rev Neurosci. 2006;7(2):137-51. doi: 10.1038/nrn1846, PMID 16429123.

43. Al Harbi KS. Treatment resistant depression: therapeutic trends challenges and future directions. Patient Prefer Adherence. 2012;6:369-88. doi: 10.2147/PPA.S29716, PMID 22654508.

44. Berton O, Nestler EJ. New approaches to antidepressant drug discovery: beyond monoamines. Nat Rev Neurosci. 2006;7(2):137-51. doi: 10.1038/nrn1846, PMID 16429123.